PCR Reagent
PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.
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WaterCAS: 7732-18-5 Formula: H2O Molecular Weight: 18.02PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.Out of Stock Item #: W433882View ProductPricing & Pack SizesTechnical Identifiers
- IUPAC Name
- oxidane
- SMILES
- [16OH2]
- InChIKey
- XLYOFNOQVPJJNP-UHFFFAOYSA-N
- InChI
- 1S/H2O/h1H2
- Synonyms
- ECOLAB Eye Wash | SalinaaxEye Wash | Water, distilled | Deionized water | Ocufresh | Water, sterile purified | AeroWa...
BsaISuitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. for DNA synthesis ? DNA-synthesis grade — high purity reagents for oligonucleotide synthesis. Use in phosphoramidite DNA synthesis where purity drives yield. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 20 U/μlOut of Stock Item #: L359050View ProductPricing & Pack SizesTechnical Identifiers
- Synonyms
- Eco31I | BsaI
- NruILiquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. for DNA synthesis ? DNA-synthesis grade — high purity reagents for oligonucleotide synthesis. Use in phosphoramidite DNA synthesis where purity drives yield. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 20 U/μlOut of Stock Item #: L398135View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- NruI | Bsp68I | BtuMI | RruI
- SalILiquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. for DNA synthesis ? DNA-synthesis grade — high purity reagents for oligonucleotide synthesis. Use in phosphoramidite DNA synthesis where purity drives yield. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. for Southern blotting ? Southern-blot grade — suited to DNA transfer and hybridization. Use in Southern blots where DNA integrity and low background matter. 20 U/μlOut of Stock Item #: L397686View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- SalI
- Human Genomic DNALiquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. For PCR ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. 200ng/μLIn Stock Item #: H669994View ProductPricing & Pack Sizes
Technical Identifiers
- Annealing Buffer for RNA Oligos (10X)Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. RNase free ? RNase-free — verified without RNase activity to protect fragile RNA. Use in RNA extraction, RT-PCR, and any RNA-sensitive workflow. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 10XOut of Stock Item #: A743818View ProductPricing & Pack Sizes
Technical Identifiers
- Annealing Buffer for DNA Oligos (10X)Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 10XOut of Stock Item #: A743817View ProductPricing & Pack Sizes
Technical Identifiers
- BBiotin-16-UTP (10mM)Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥95% 10mMIn Stock Item #: B744152View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- Biotin-16-UTP | Biotin-16-(5-aminoallyl)-UTP；Biotin-16-Aminoallyluridine-5'-Triphosphate | Biotinyl-ε-aminocaproyl-γ-...
- RNase RLiquid Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Endotoxin tested ? Endotoxin-tested — each lot assayed and labeled with its endotoxin level. Use when you need a documented endotoxin value for risk assessment. Mycoplasma free ? Mycoplasma-free — tested negative for mycoplasma contamination. Use in cell culture to prevent silent infections that skew experiments. for DNA synthesis ? DNA-synthesis grade — high purity reagents for oligonucleotide synthesis. Use in phosphoramidite DNA synthesis where purity drives yield. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Endonuclease ? Endonuclease-free — without internal-cleaving nuclease activity. Use to protect DNA/RNA from internal strand cleavage during prep. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. Exonuclease ? Exonuclease-free — without end-trimming nuclease activity. Use to preserve nucleic-acid ends in sensitive molecular procedures. DNase free ? DNase-free — verified without DNase activity to protect DNA. Use whenever DNA integrity must be preserved during handling or storage. ≥95%(SDS-PAGE) expressed in E.coli;20 U/μlOut of Stock Item #: R749973View ProductPricing & Pack Sizes
Technical Identifiers
- Species
- E. coli
- Accession #
- P21499
- Expression system
- E. coli
- Bioactivity
- 20 U/μl
- Synonyms
- RNase R | Ribonuclease R
- Carrier RNA (Poly A)CAS: 26763-19-9 Formula: C10H13KN5O7P Molecular Weight: 385.31Solid BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥99%Out of Stock Item #: C744418View ProductPricing & Pack Sizes
Technical Identifiers
- IUPAC Name
- potassium;[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate
- SMILES
- C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)[O-])O)O)N.[K+]
- InChIKey
- WADAUMZGCLRVDM-MCDZGGTQSA-M
- InChI
- show more
- NTP Mix (10mM each, Tris Buffered)Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥99% 10mMOut of Stock Item #: N748678View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- NTP Mixture；NTP Mix
- NTP set (100mM each, Tris Buffered)Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥99% 100mMOut of Stock Item #: N748680View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- NTP Set Solution | NTP Set Solution 100mM
Frequently Asked Questions — PCR Reagent
What does PCR Reagent mean?
PCR Reagent indicates that the product has been processed and tested for PCR, sequencing and nucleic acid amplification. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.
Is PCR Reagent certified DNase / RNase / protease free?
Many molecular biology grades include nuclease activity testing. The exact tests and limits are listed on the Certificate of Analysis. For applications sensitive to nuclease contamination (qPCR, NGS library prep, mRNA work), prefer grades explicitly labeled DNase free, RNase free or Protease Free.
Can I use PCR Reagent for sensitive workflows like qPCR or NGS?
Yes, where the product is qualified for the workflow. For PCR / qPCR, look for For PCR or PCR Reagent grade. For NGS sample prep, prefer molecular biology, RNase free or BioReagent grade — and verify lot QC data.
How does PCR Reagent differ from analytical reagent grades?
Analytical grades focus on chemical purity. Molecular biology grades add tests directly relevant to nucleic-acid and protein workflows — nuclease activity, microbial limits, sometimes endotoxin. Use molecular-biology grades whenever a downstream enzyme or biological readout is involved.
What documentation is provided?
Certificate of Analysis with QC test results, SDS, and where applicable lot-specific functional or activity data. Application notes and protocol guidance are available on the product page.