GPCR GPR120 Antibody - Primary antibody, specific to GPCR GPR120, Rabbit IgG

Cat. No.: Ab106082

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human GPCR GPR120 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.
Application:
           WB: 1/500-1/1000
           IHC: 1/50-1/100 

           ICC/IF: 1/50-1/200
Protein Function：
Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects particulary in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediates the taste of fatty acids.

Specifications

Product Name

GPCR GPR120 Antibody - Primary antibody, specific to GPCR GPR120, Rabbit IgG

Synonyms

PGR4 antibody | PGR 4 antibody | Omega-3 fatty acid receptor 1 antibody | O3FAR1 antibody | O3FA1_HUMAN antibody | MGC119984 antibody | HGNC:19345 antibody | PGR4 | GT01 | OB10Q | BMIQ10 | GPR120 | GT01 antibody | GPR129 antibody | GPR120 antibody | GPR12

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Host species

Rabbit

Specificity

GPCR GPR120

Immunogen

A synthetic peptide derived from human GPR120

Positive Control

WB: HT-29 and A549 cell lysates. IHC: Human prostate tissue. ICC/IF: LO2 cells.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: GPCR GPR120 Antibody (Ab106082)

GPCR GPR120 Antibody (Ab106082) - Western Blot
All lanes: GPCR GPR120 Antibody (Ab106082) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 57, 43 kDa
Exposure time: 75.5 s

IHC: GPCR GPR120 Antibody (Ab106082)

GPCR GPR120 Antibody (Ab106082) - IHC
Immunohistochemical analysis of GPR120 staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GPCR GPR120 Antibody (Ab106082) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF: GPCR GPR120 Antibody (Ab106082)

GPCR GPR120 Antibody (Ab106082) - IF/ICC
Immunofluorescent analysis of GPR120 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GPCR GPR120 Antibody (Ab106082) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

• Application: Dilution info
• IF/ICC:

  1/50-1/200 ;

• WB:

  1/500-1/1000 ;

• IHC:

  1/50-1/100 ;

Associated Targets(Human)

FFAR4 Tchem Free fatty acid receptor 4 (0 Activities)

Discover Target: FFAR4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action