Hanks′ Balanced Salt solution - for cell culture, liquid,With sodium bicarbonate,sterile-filtered,for cell culture

Cat. No.: H477259

GRADE & PURITY for Cell culture, liquid,With sodium bicarbonate,sterile-filtered,for cell culture

Storage

Room temperature

Specifications

Specifications & Purity

for cell culture, liquid,With sodium bicarbonate,sterile-filtered,for cell culture

Storage

Room temperature

Grade

for Cell culture

Application Protocols

The following are general, literature-based procedures; they are not validated protocols for SKU H477259. Adjust to your system and consult the CoA for composition.

• Cell washing (adherent cells):
  1. Aspirate medium; 2) Gently add prewarmed HBSS to cover cells; 3) Rock 30–60 s; 4) Aspirate; repeat as needed. For enzyme addition, use HBSS without Ca2+/Mg2+ prior to EDTA/trypsin.
• Cell washing (suspension cells):
  1. Add 5–10× volume HBSS; 2) Centrifuge at 200–400 × g for 5 min; 3) Aspirate supernatant; 4) Resuspend pellet in fresh HBSS or medium.
• Tissue handling:
  • Keep HBSS chilled (4 °C) during dissection to slow metabolism, unless enzyme activity is required, in which case use 37 °C.
• Dye/indicator preparation:
  • Dissolve water-soluble probes in HBSS immediately before use; filter-sterilize if needed (0.22 µm). Avoid divalent cations if they interfere with chelator-based assays.
• pH stabilization:
  • Equilibrate bicarbonate-containing HBSS in 5% CO2 for 15–30 min before long incubations; for ambient work, use HEPES-supplemented systems.

Maintain aseptic technique; minimize bottle openings and consider aliquoting to reduce contamination risk.

Biological Roles

HBSS supports physiological function during cell and tissue manipulation by supplying ions and buffering capacity (general, literature):

• Sodium and potassium: Maintain transmembrane gradients essential for cell volume and membrane potential.
• Chloride: Primary counter-ion balancing cationic charge; influences membrane transport and pH regulation.
• Calcium and magnesium (if present): Modulate cell adhesion, integrin function, enzyme activities, and membrane stability.
• Phosphate/bicarbonate buffers: Stabilize extracellular pH near physiological values. Bicarbonate requires CO2 equilibrium; phosphate provides ambient buffering.
• D-Glucose (if present): Supplies a readily metabolizable substrate for short-term energy demands during handling.

Use cases benefiting from these roles

• Preserving cell integrity during washing/centrifugation.
• Supporting viability during short incubations outside complete media.
• Providing a defined ionic environment for receptor signaling assays, calcium-flux measurements, and transporter studies.

Note: The exact biological behavior depends on the precise formulation (e.g., presence/absence of divalent cations or glucose). Refer to the item’s CoA for composition details for SKU H477259.

Buffer Applications

HBSS is a balanced salt solution used as a physiological buffer for cell culture workflows (general, literature):

• Buffer systems: Typically combines bicarbonate (CO2-dependent) and phosphate buffering. In a 5% CO2 incubator, bicarbonate equilibrates to maintain pH ~7.2–7.4. Phosphate provides modest buffering in ambient air.
• Practical use:
  • Equilibrate bicarbonate-containing HBSS in a CO2 incubator for ≥15–30 min prior to long incubations.
  • For non-CO2 environments or microscopy outside the incubator, consider HEPES-supplemented variants or phosphate-dominant buffers to stabilize pH.
• Applications:
  • Cell washing, reagent preparation, and short-term incubations without serum.
  • Matrix/enzyme preparation where defined ionic strength is required.
• Compatibility:
  • Enzyme work: Choose Ca2+/Mg2+ content to match enzyme/cofactor needs.
  • Chelators (e.g., EDTA) will sequester divalent cations; use HBSS without Ca2+/Mg2+ when chelators are present.

All details are general guidance; composition and buffering capacity for SKU H477259 should be confirmed on the CoA/Spec Sheet.

Green Alternatives

For cell-handling solutions, “green” considerations focus on formulation simplicity, packaging, and transport rather than solvent replacement.

• Comparators:
  • HBSS vs PBS/DPBS: PBS is compositionally simpler (fewer salts, no bicarbonate), which can reduce raw material footprint, but may be less supportive for extended ex vivo handling. HBSS may reduce need for media during short procedures, potentially lowering resource use.
  • HBSS vs Ringer’s/Lactated Ringer’s: Ringer-type solutions offer alternative buffering/anion systems; selection depends on biological compatibility and assay needs.

Sustainability considerations (general)

• Packaging: Choose bulk containers or bag-in-box to reduce plastic mass per liter; select recyclable materials when available.
• Storage and shipping: Ambient-stable HBSS avoids cold-chain energy use compared to some specialized buffers.
• On-site preparation: For high-volume users, preparing from sterile concentrates or powders can minimize shipping weight and packaging waste; ensure validated sterility and quality controls.

These are general best practices; no item-specific environmental claims are made for this SKU.

Pharmaceutical Uses

This product is intended for research use only (per Product Data) and is not for human or animal therapeutic use.

General formulation/manufacturing context (literature)

• Balanced salt solutions analogous to HBSS may be used as process buffers or wash solutions in bioprocessing, cell banking, and device rinsing steps.
• Attributes valued in such contexts include isotonicity, defined ionic strength, low endotoxin and bioburden, and chemical clarity (e.g., phenol red-free for optical monitoring).
• Regulatory status: Any use in GMP manufacturing would require qualification of supplier, lot-specific testing, and adherence to pharmacopeial or internal specifications; none are specified for this item.

For SKU H477259, no pharmacopeial compliance or manufacturing suitability claims are specified; consult the CoA/Spec Sheet if considering regulated applications.

Physical Properties

Item-specific (Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet. (Mixture)

General/literature values for HBSS (typical ranges; not item-specific)

• Physical state: Clear, aqueous solution (if supplied ready-to-use); color may be water-white to light red when phenol red is included.
• pH (ambient, equilibrated with 5% CO2 if bicarbonate-buffered): ~7.1–7.5 (literature).
• Osmolality: ~260–320 mOsm/kg (literature), formulation dependent.
• Density: ~1.00–1.01 g/mL at 20–25 °C (aqueous salt solution; literature).
• Conductivity: High (tens of mS/cm; literature) due to millimolar salt content.
• Solubility: Fully miscible with water; not miscible with most organic solvents.

Practical notes

• Bicarbonate-containing HBSS will show pH drift with atmospheric CO2; equilibrate in a CO2 incubator to reach nominal pH.
• Divalent cation content (Ca2+/Mg2+) materially affects cell adhesion and enzymatic dissociation; verify variant before use.

All numeric values above are typical literature ranges and are not specifications for this item.

Quality & Grades

Item-specific (Product Data)

• Grade: for Cell culture
• Sterility/endotoxin/heavy metal/bioburden/particulate limits: Not specified for this item; refer to CoA/Spec Sheet.

What “for Cell culture” typically implies (general guidance)

• Manufactured to be compatible with mammalian cell handling: sterile filtration and low particulate burden; low bioburden; controlled pH/osmolality.
• Often tested for: sterility (per pharmacopeial methods), endotoxin (e.g., LAL), and pH/osmolality within defined windows. Exact acceptance criteria vary by supplier.
• Additives/variants: May be supplied with/without Ca2+/Mg2+, with/without phenol red, and with/without D-glucose. Choice should match application (e.g., enzymatic dissociation typically prefers no Ca2+/Mg2+).

Analytical considerations

• For downstream analytical work (e.g., LC–MS on secretome), phenol red-free and low-fluorescence variants reduce background.
• Trace metal content and residual organics can affect sensitive cell types; verify CoA if critical.

Refer to the specific CoA for this SKU for exact quality attributes and release specifications.

Reaction & Applications

HBSS is used for cell and tissue handling rather than chemical synthesis. Key laboratory applications (general, literature):

• Cell washing and resuspension: Maintains osmotic balance during centrifugation/wash steps, minimizing cell stress.
• Tissue dissection/transport: Chilled HBSS supports temporary maintenance of excised tissues prior to processing.
• Reagent vehicle: Diluent for water-soluble dyes, Ca2+-sensitive indicators, and assay substrates when serum-free conditions are required.
• Enzymatic dissociation workflows:
  • Use HBSS without Ca2+/Mg2+ in combination with EDTA or proteolytic enzymes (e.g., trypsin, collagenase) to improve cell detachment.
  • Reintroduce Ca2+/Mg2+ post-dissociation for cell recovery and adhesion.
• Imaging/assay compatibility: Phenol red-free variants reduce background in absorbance/fluorescence assays; glucose-containing HBSS can support short-term metabolic needs during handling.
• Viral transduction/electroporation: Often used as an isotonic wash or equilibration buffer before/after the procedure (confirm protocol-specific requirements).

Practical tips

• Equilibrate bicarbonate-buffered HBSS in 5% CO2 for stable physiological pH during extended manipulations.
• Keep sterile; warm to 20–37 °C as needed to avoid thermal shock to cells.

All points are general for HBSS; consult the item CoA for exact composition relevant to your protocol.

Reaction Conditions

As a physiological buffer, HBSS is used under conditions suitable for cells and enzymes rather than chemical reactions. General guidance (literature):

• Temperature: Typically used at 20–37 °C depending on cell type and workflow. Warm to 37 °C to avoid thermal shock during cell handling.
• Atmosphere: For bicarbonate-containing HBSS, 5% CO2 equilibration stabilizes pH near 7.2–7.4. For work outside CO2, use phosphate-dominant or HEPES-supplemented buffers.
• Additives: Serum proteins, glucose, divalent cations, and chelators (EDTA) should be chosen to match the protocol (e.g., dissociation vs adhesion).
• Contact times: Minutes to hours for typical wash or incubation steps; prolonged incubation without nutrients may reduce viability.
• Compatibility: Many dyes and indicators are validated in HBSS; check reagent datasheets for salt and pH sensitivity.

These conditions are illustrative and not specifications for SKU H477259. Consult your protocol and the item’s CoA for composition-specific constraints.

Safety & Handling

Item-specific (Product Data)

• GHS Classification, Signal Word, Pictograms, H-Statements: Not specified for this item; consult the SDS for authoritative safety information.
• Storage: Room temperature (per Product Data). Protect from microbial contamination; keep container tightly closed.

General safety guidance (aqueous balanced salt solutions; literature/practice)

• Likely low hazard under normal laboratory use; nonetheless, handle using good laboratory practice.
• Recommended PPE: lab coat, nitrile gloves, safety glasses. Use aseptic technique for cell culture applications.
• Incompatibilities: Avoid mixing with reactive chemicals (strong acids/bases outside buffer capacity, oxidizers). Do not autoclave sealed bottles without confirming suitability; heat may alter pH and phenol red.
• First aid (general): If skin/eye contact occurs, rinse with water. If ingested/inhaled in significant amount, seek medical attention. Follow institutional policies.
• Microbiological risk: Once opened, solutions can become contaminated; use sterile technique, minimize repeated bottle entry, and consider aliquoting.

Always defer to the product SDS and institutional EHS guidance for risk assessment and emergency procedures.

Solvent Selection

HBSS is not a solvent of choice for organic synthesis; it is an aqueous physiological salt solution optimized for cell and tissue handling.

• Polarity/miscibility: Highly polar; fully miscible with water; immiscible with most nonpolar organic solvents.
• Buffering system: Typically bicarbonate and/or phosphate. Bicarbonate-buffered HBSS performs best in 5% CO2 atmosphere; phosphate provides ambient buffering but with lower CO2 responsiveness.
• Isotonicity: Formulated to be isotonic with mammalian cells; suitable for washing, dilutions, reagent preparation for cell assays, and tissue transport.
• When to choose HBSS vs alternatives:
  • HBSS vs PBS/DPBS: HBSS includes balanced ionic composition and often glucose; preferred for cell viability during handling. PBS is simpler and suitable for general rinsing/fixation when divalent cations are not desired.
  • HBSS with Ca2+/Mg2+: Favors cell–cell/ECM adhesion and some enzyme activities.
  • HBSS without Ca2+/Mg2+: Preferred for EDTA/enzyme-based dissociation protocols to reduce adhesion.
• Compatibility: Suitable as vehicle for water-soluble reagents, dyes, and indicators used in live-cell assays; confirm that reagent performance is compatible with salts and pH of HBSS.

All features above are general characteristics of HBSS and not item-specific specifications.

Storage & Reconstitution

Item-specific (Product Data)

• Storage Conditions: Room temperature
• Shipped In: Not specified for this item; refer to CoA/Spec Sheet.
• Reconstitution: Not applicable for ready-to-use solutions; if supplied otherwise, Not specified for this item; refer to CoA/Spec Sheet.

General guidance (balanced salt solutions)

• Protect from contamination: Store tightly closed; use sterile technique; wipe closures with 70% IPA before opening in biosafety cabinet.
• Temperature: Many HBSS formulations are stable at ambient temperature; avoid excessive heat or freezing unless label permits. Freezing can cause salt precipitation and container damage.
• Light sensitivity: Phenol red-containing solutions may slowly photobleach; store away from direct light.
• After opening: Record first-use date; for sterile work, consider aliquoting into sterile containers. Discard if turbidity, precipitate, or color change is observed, or if sterility is compromised.
• Pre-use: Warm to room temperature or 37 °C as required by protocol; equilibrate bicarbonate-buffered HBSS in 5% CO2 for stable pH.

For SKU H477259, any specific shelf life, sterility assurance, and composition details are Not specified for this item; refer to the product label and CoA/Spec Sheet.

Structure & Identity

Item-specific (Product Data)

• Product: Hanks′ Balanced Salt solution (HBSS), SKU: H477259
• Grade: for Cell culture
• Storage condition: Room temperature
• CAS, InChIKey, SMILES, Molecular Formula, Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

General description (literature)

• HBSS is an isotonic, inorganic salt solution formulated to maintain physiological pH and osmotic balance for handling mammalian cells/tissues outside complete growth media.
• Typical components (formulation-dependent) include NaCl, KCl, CaCl2, MgSO4 or MgCl2, Na2HPO4/NaH2PO4, NaHCO3, and optionally D-glucose and phenol red. Variants exist “with” or “without” Ca2+/Mg2+ depending on downstream use (e.g., chelator- or enzyme-based cell dissociation).
• Structure: Mixture (no single defined molecular structure). Functionally, it provides Na+/K+ gradients, chloride counter-ions, bicarbonate/phosphate buffering, and divalent cations for adhesion and enzyme activity (when present).

Notes

• Because HBSS is a mixture, identifiers such as a single CAS number or InChIKey are not applicable unless tied to a specific supplier formulation. Always consult the item’s CoA/SDS for exact composition and identifiers.

Synthetic Utility

HBSS is not typically used in synthetic organic or inorganic chemistry. It is an aqueous physiological salt solution intended for biological workflows.

• If an aqueous medium is required for bioorthogonal reactions or enzyme-catalyzed transformations, ensure the ionic strength, pH, and divalent cations in HBSS are compatible with the catalyst/enzyme system.
• For most chemical syntheses, use defined buffers (e.g., phosphate, Tris, HEPES) or deionized water to avoid unintended ionic effects.

For synthetic applications, refer instead to the Reaction & Applications and Buffer Applications sections for biological handling considerations.

Target Specificity

Not applicable. HBSS is a balanced salt solution and does not possess antigen/epitope specificity, clone, or isotype attributes.

For application-relevant details, refer to Reaction & Applications and Buffer Applications.