GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Carrier Free, ExactAb™, Validated, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Histone H3 (tri methyl K4) Antibody, Polyclonal, could be used for WB, IHC, ELISA and so on.
Specifications
Product Name
Histone H3 (tri methyl K4) Antibody - Primary antibody, specific to Histone H3 (tri methyl K4)
Synonyms
H3 histone family member E pseudogene antibody | H3 histone family, member A antibody | H3/A antibody | H31_HUMAN antibody | H3F3 antibody | H3FA antibody | Hist1h3a antibody | HIST1H3B antibody | HIST1H3C antibody | HIST1H3D antibody | HIST1H3E antibody
Specifications & Purity
Carrier Free, ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replic
Host species
Rabbit
Specificity
Histone H3 (tri methyl K4)
Immunogen
KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K4 of human Histone H3 protein (AA 2-32).
Positive Control
WB: Mouse skeletal muscle, Rat skeletal muscle, HeLa, A549, K562 and Jurkat lysates. IHC: Human breast cancer tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Histone H3 (tri methyl K4) Antibody (Ab107957) - Western Blot All lanes: Histone H3 (tri methyl K4) Antibody (Ab107957) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa Observed band size: 17 kDa Exposure time: 25.4 s
Histone H3 (tri methyl K4) Antibody (Ab107957) - Western Blot All lanes: Histone H3 (tri methyl K4) Antibody (Ab107957) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa Observed band size: 17 kDa Exposure time: 21.1 s
Histone H3 (tri methyl K4) Antibody (Ab107957) - IHC Immunohistochemical analysis of Histone H3 (TriMethyl-K4) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Histone H3 (tri methyl K4) Antibody (Ab107957) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Histone H3 (tri methyl K4) Antibody (Ab107957) - ELISA Direct ELISA antibody dose-response curve using Histone H3 (tri methyl K4) Antibody (Ab107957). Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 µg/mL. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Application
Application
Dilution info
IHC
1/50-1/200
WB
1/500-1/1000
ELISA
Use at an assay dependent concentration.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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