HRP-StabilPLUS - Ready-to-use (RTU)

Cat. No.: H494387

GRADE & PURITY Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Overview

Product Characteristics 

HRP-StabilPLUS is used for the dilution and stabilization of Horseradish Peroxidase (HRP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enables the making of re-diluted, ready-to-use HRP-conjugates, minimizing assay errors in dilution. 

Superior stabilization of HRP conjugated antibodies in low as well as high protein dilutions is seen, when using HRP-StabilPLUS. Diluted HRPconjugated proteins display stability as follows:

• at least 4 years at 2-8 °C 

• at least 2 years at room temperature 

• at least 10 weeks at 37 °C.

Composition & Properties 

HRP-StabilPLUS is a ready-to-use buffer that appears as a slightly white solution. The product is based on a mild acid Tris buffer containing proprietary stabilizing components. HRP-StabilPLUS contains neither BSA, nor other material from bovine serum, azide, mercury or other toxic components.

Working Procedure 

1.Make a series of dilutions of the HRP-conjugated protein in HRP-StabilPLUS in order to determine the optimal dilution. 

2.Run the assay as usual or store the diluted HRPconjugated protein preferably at 2-8 °C.

Tips & Tricks 

• HRP-StabilPLUS cannot be used for the dilution of antibodies directed against rabbit immunoglobulins i.e. Swine anti-Rabbit Ig & Goat anti-Rabbit Ig etc. UNI-StabilPLUS (cat. no. U494463) is recommended for this purpose.

Handling & Storage 

• Store solution at 2-8 °C

Specifications

Specifications & Purity

Ready-to-use (RTU)

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: Light sensitive

Application Protocols

Tested applications, recommended dilutions, and step‑by‑step protocols are not specified for this item. Refer to the product CoA/Spec Sheet and your laboratory’s validated SOPs.

General practice (context only; not item‑specific instructions):

• Prepare HRP‑conjugate working solutions immediately before use, or store aliquots at 2–8°C in a suitable stabilizer validated for your assay.
• Confirm compatibility with your chosen detection substrate (e.g., TMB for ELISA, luminol for chemiluminescence) by running control titrations and matrix blanks.

Follow your internal assay development guidelines for optimization of incubation times, temperatures, and wash buffers.

Biological Roles

The product itself is not a biological macromolecule with intrinsic cellular roles; rather, it stabilizes horseradish peroxidase (HRP) used as a reporter enzyme in immunochemical assays.

Context on HRP (literature/general):

• HRP is a plant heme peroxidase that catalyzes the reduction of hydrogen peroxide with concomitant oxidation of various electron donors (chromogenic or chemiluminescent substrates). The catalytic cycle involves Compound I/II intermediates with ferryl heme and a porphyrin or protein radical.
• In bioanalytical workflows, HRP is conjugated to antibodies, streptavidin, or other binders to transduce binding events into measurable colorimetric (e.g., TMB, OPD), fluorogenic (e.g., Amplex substrates), or chemiluminescent (e.g., luminol) signals.

Role of stabilizers (literature/general):

• Maintain tertiary/quaternary structure and protect the heme center against oxidative inactivation.
• Prevent aggregation and surface denaturation, especially during storage and low‑concentration handling in polystyrene plates or polypropylene tubes.
• Buffer pH and ionic strength to sustain activity and reduce background signals in assay matrices.

HRP‑StabilPLUS is intended for research use only and has no therapeutic or endogenous biological role. For composition‑specific biological compatibility (e.g., presence/absence of animal‑derived components), consult the CoA/Spec Sheet.

Buffer Applications

HRP‑StabilPLUS functions as a stabilizing and potentially diluting buffer for HRP enzyme conjugates in immunoassays. Item‑specific buffer composition, pH, and recipes are not provided for this product.

Typical uses in assay buffers (general):

• Conjugate diluent: Maintain HRP‑labeled antibodies/streptavidin at working concentrations while minimizing nonspecific adsorption to plastics and preserving activity across assay runs.
• Storage medium: Support stability of HRP conjugates at 2–8°C during real‑time storage and short bench‑top exposure; aliquoting is recommended to avoid repeated freeze–thaw (general).
• Background control: Balanced surfactant and blocking agents can reduce off‑target binding and matrix effects in ELISA/Western blot; verify compatibility with your blocking buffer and substrate chemistry.

Practical considerations:

• Avoid mixing with buffers containing HRP inhibitors (e.g., azide) or high concentrations of primary amines if using amine‑reactive coupling steps immediately afterward (general guidance).
• Validate final buffer composition for downstream detection (TMB/HRP or luminol systems) to ensure no quenching, precipitation, or pH drift during incubation.

For exact buffer parameters, dilution instructions, and compatibility declarations for HRP‑StabilPLUS, refer to the product CoA/Spec Sheet and your internal assay SOPs.

Green Alternatives

HRP‑StabilPLUS is an aqueous formulation intended for biological assay workflows, which generally aligns with green chemistry principles by avoiding volatile organic solvents and hazardous reagents.

General sustainability considerations for HRP stabilizers:

• Solvent system: Water‑based buffers minimize VOC emissions and flammability risk versus organic cosolvent systems (general).
• Preservatives: Choice of preservative affects environmental fate; azide is effective at low levels but is toxic to aquatic life and inhibits HRP. Alternative biocides (e.g., isothiazolinones) and sterile filtration can reduce environmental impact, but must be validated for HRP compatibility (literature/general; not specified for this item).
• Protein‑free vs. protein‑containing formulations: Protein‑free systems avoid animal‑derived raw materials, supporting supply chain and regulatory preferences in some labs (general).

Comparison (general; not item‑specific):

• Aqueous HRP stabilizer vs. glycerol‑rich storage: Glycerol reduces freezing but increases viscosity and waste COD; aqueous systems at 2–8°C reduce organic load but may require preservatives.
• Azide‑preserved vs. azide‑free: Azide preserves effectively but inhibits HRP and presents disposal concerns; azide‑free systems improve compatibility with HRP and wastewater treatment but may have shorter open‑vial shelf life without sterile technique.

Best practices:

• Use only the volume needed, maintain clean technique to reduce preservative demand, and dispose according to local regulations. Validate greener preservative systems in your assay before broad adoption.

Pharmaceutical Uses

Not applicable. HRP‑StabilPLUS is supplied strictly for research use only and is not intended for human or veterinary diagnostic, therapeutic, or GMP manufacturing applications.

General information (context only):

• Enzyme stabilizers similar in concept are used in R&D analytics and quality control assays (e.g., ELISA) to monitor bioprocesses. However, transitioning a research‑grade stabilizer to regulated manufacturing requires documented composition, validated performance, impurity profiles, and change control — none of which are specified for this item.
• If your laboratory operates under GLP/GMP, confirm suitability via vendor qualification and request any available quality statements or custom documentation. Otherwise, employ this product for non‑clinical research and method development only.

No pharmacopeial status, excipient designation, or clinical formulation role is specified for this item.

Physical Properties

Item-specific physicochemical specifications have not been provided for HRP‑StabilPLUS.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• pH: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, osmolality: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: As a ready-to-use stabilizing reagent, it is generally supplied as an aqueous solution (general information); exact composition/solvent system is not specified for this item.
• Freezing point/boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Preservatives or proteinaceous stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/general):

• HRP stabilizers are typically buffered in the near‑neutral range to preserve peroxidase activity and minimize acid/base denaturation (literature).
• Inclusion of chelators can suppress metal‑catalyzed peroxide decomposition and minimize free radical damage to HRP (literature).
• Polyols, sugars, amino acids, or polymers can enhance thermal and long‑term stability via preferential hydration and crowding effects (literature).

Because this is a formulated mixture, traditional single‑compound parameters (e.g., logP, SMILES) do not apply. For method development or QC, consult the product’s CoA/Spec Sheet for any available numeric specifications relevant to your assay (e.g., UV background, conductivity, or pH window), and validate under your specific storage and assay conditions.

Quality & Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Context for this product class (general):

• HRP stabilizers are manufactured under controlled conditions to ensure low background in colorimetric and chemiluminescent assays (e.g., ELISA, Western blot, IHC). Key quality attributes commonly evaluated include low UV/visible background, minimal lot‑to‑lot variation in pH/ionic strength, absence of substances that inhibit HRP, and preservative efficacy for extended shelf‑life (literature/general).
• For conjugate diluent/stabilizer applications, end‑use performance is typically characterized by retention of HRP activity after thermal and real‑time aging, maintenance of signal/noise in representative assays, and absence of precipitates upon storage at 2–8°C (general).
• If the formulation is protein‑free or BSA‑containing, this affects blocking behavior and compatibility with downstream analyses (e.g., mass spectrometry, anti‑mouse secondary cross‑reactivity). The presence/absence of animal‑derived components should be confirmed in the CoA if relevant to your application (not specified for this item).

Recommendations:

• Verify that the lot’s CoA meets your method’s acceptance criteria (e.g., appearance clarity, pH window, microbial limits, and functional stability of a test HRP conjugate).
• Run a side‑by‑side stability comparison using your HRP conjugate(s) under accelerated conditions to qualify the lot for critical assays.

Reaction & Applications

Although not a reagent for chemical synthesis, HRP‑StabilPLUS is designed to support the enzymatic reaction performance of horseradish peroxidase by preserving the native structure and activity of HRP conjugates in immunoassays and blotting protocols.

Typical applications (general, for HRP stabilizers):

• Long‑term and accelerated stability of HRP‑conjugated antibodies, proteins (e.g., streptavidin‑HRP), and polymers used in ELISA, Western blot, dot blot, and IHC/ICC.
• Working diluent for HRP conjugates to reduce non‑specific adsorption to plastics and minimize background.
• Short‑term storage of enzyme–substrate working mixes where permitted by assay design, minimizing drift in activity (validate experimentally).

Mechanistic considerations (literature/general):

• Preferential hydration and kosmotropic effects from polyols/sugars/amino acids stabilize HRP’s tertiary structure; chelators limit metal‑catalyzed peroxide decomposition; surfactants suppress aggregation and surface denaturation; optimized ionic strength/pH preserves the heme environment and catalytic cycle.

Practical tips:

• Minimize exposure to light and elevated temperatures; HRP activity can decline via heme oxidation and radical damage.
• Avoid repeated freeze–thaw cycles for HRP conjugates; store aliquots in stabilizer when possible.
• Validate compatibility with your detection chemistry (TMB, OPD, luminol) to ensure no quenching or background elevation.

Item‑specific application claims are not provided; consult the CoA/Spec Sheet and perform method‑specific qualification.

Reaction Conditions

Item‑specific usage conditions are not provided. The following general guidance applies to HRP‑based assays and conjugate handling (literature/general):

• Temperature: Maintain HRP conjugates and working solutions at 2–8°C when feasible; equilibrate to room temperature during assay incubations. Avoid prolonged exposure to >25–30°C unless validated.
• Light: Protect from light to minimize photo‑oxidative damage to heme and substrates (e.g., TMB, luminol).
• pH: HRP typically exhibits robust activity near neutral pH for common substrates; strongly acidic/basic conditions can inactivate the enzyme or alter substrate spectra. Final pH should match your detection chemistry’s recommended range.
• Ions and metals: Transition metals can catalyze peroxide decomposition and generate radicals; chelators are often beneficial but must be balanced to avoid interfering with assay components.
• Organic cosolvents: Keep concentrations low; validate tolerance as even modest percentages can diminish HRP activity.
• Substrate compatibility: Confirm that the stabilizer does not quench chromogenic/chemiluminescent readouts or increase background; run matrix blanks and spike‑recoveries.

For HRP‑StabilPLUS specifically, refer to the CoA/Spec Sheet for any recommended incubation times, compatible substrates, or dilution factors, and qualify in your assay matrix.

Safety & Handling

Safety information specific to HRP‑StabilPLUS has not been provided.

• GHS classification, pictograms, signal word, H‑statements: Not specified for this item; refer to SDS.
• Primary hazards: Typically low for aqueous stabilizers; however, formulations may contain preservatives or surfactants that pose irritation or sensitization risks (general). Handle as a laboratory chemical of unknown hazard.
• PPE: Wear appropriate lab coat, safety glasses, and nitrile gloves. Use in a well‑ventilated area; avoid inhalation of aerosols and contact with skin/eyes.
• Storage incompatibilities: Keep away from strong oxidizers or reducers until compatibility is confirmed. Avoid microbial contamination of the container. Do not mix with solutions containing metals or peroxides unless validated for your assay (general guidance).
• Special risks for peroxidase workflows: Sodium azide inhibits HRP activity; ensure compatibility of any azide‑containing solutions used upstream/downstream in your workflow (general/literature note; formulation content for this item is not specified).
• First aid (overview): In case of eye/skin contact, rinse with water for at least 15 minutes. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical attention. Provide SDS to healthcare personnel.
• Spill/cleanup: Absorb with inert material, collect, and rinse area with water. Prevent release to drains if formulation contains preservatives. Dispose according to institutional and local regulations.

Always consult the product SDS for authoritative hazard, toxicology, and disposal guidance.

Solvent Selection

This product is a formulated aqueous reagent rather than a solvent. Traditional solvent selection criteria (polarity, dielectric constant, miscibility) are not directly applicable.

Practical guidance (general):

• Use HRP‑StabilPLUS as provided (ready‑to‑use) unless your validated SOP specifies dilution or mixing with other assay buffers. If dilution is required, use high‑purity water or a buffer system known to be compatible with HRP and your detection chemistry; avoid introducing agents known to inhibit HRP (e.g., azide) unless intentionally used and validated.
• When preparing working solutions of HRP‑conjugated antibodies or streptavidin, ensure the final buffer composition preserves enzyme activity and minimizes non‑specific binding. Stabilizer formulations often double as diluents to maintain signal over multiple freeze‑thaw or bench‑top cycles (general).
• If your assay requires organic cosolvents (e.g., for hydrophobic substrates), validate that their final concentrations do not compromise HRP activity. Short‑chain alcohols and DMSO can reduce peroxidase activity at elevated percentages (literature/general).

For composition‑specific compatibility and dilution guidance, refer to the CoA/Spec Sheet for this item and validate in your workflow.

Storage & Reconstitution

• Storage conditions (item‑specific): Store at 2–8°C, protected from light.
• Shipping: Shipped on wet ice.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If supplied as a liquid (common for stabilizers), use as provided and avoid contamination by practicing aseptic technique. Do not assume lyophilization or concentrate status without documentation.
• Handling guidance (general):
  • Allow the container to equilibrate to room temperature before opening to minimize condensation.
  • Mix gently by inversion; avoid vigorous foaming which can denature proteins in your conjugate when mixing downstream.
  • Dispense using clean, dedicated pipettes to prevent microbial introduction; recap promptly.
• Freeze–thaw: Unless explicitly supported by the CoA, avoid freezing; repeated freeze–thaw of HRP conjugates can reduce activity. Store working aliquots at 2–8°C and protect from light during use.
• Stability after opening: Not specified for this item; establish in‑use dating within your lab’s QA system based on microbial controls and performance trending.

For definitive guidance on shelf life, in‑use stability, and any dilution instructions specific to HRP‑StabilPLUS, consult the CoA/Spec Sheet and the product SDS.

Structure & Identity

HRP-StabilPLUS is a formulated stabilizing reagent intended for horseradish peroxidase (HRP) enzyme conjugates and HRP-containing assay solutions. It is a proprietary mixture; no discrete small‑molecule identity applies.

• Product type: Aqueous HRP stabilizer/diluent (formulated mixture)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChI / InChIKey: Not applicable for proprietary mixtures; not specified for this item.
• Structural features (descriptive): Typically such stabilizers comprise buffering agents, protein/peptide or polymeric protectants, chelators, surfactants, and preservatives to maintain HRP tertiary structure and catalytic competence; the exact composition for HRP‑StabilPLUS is not specified.
• Physical form: Not specified for this item; refer to CoA/Spec Sheet.

2D structural description: Not applicable — HRP-StabilPLUS is not a single chemical entity but a multi‑component aqueous formulation designed to reduce denaturation, aggregation, proteolysis, and metal‑catalyzed degradation of HRP conjugates during storage and use.

Synthetic Utility

Not applicable. HRP‑StabilPLUS is a biological assay reagent for stabilizing HRP enzyme conjugates, not a building block or reagent for organic or inorganic synthesis.

Guidance for chemists designing assays (general):

• Treat the stabilizer as part of the reaction medium for the HRP‑catalyzed reporting reaction. Validate that it does not alter substrate kinetics (e.g., TMB, OPD, luminol) or interfere with metal‑dependent quenchers/enhancers sometimes used in chemiluminescent systems.
• If coupling chemistry (e.g., NHS esters, maleimides) is performed upstream to generate HRP conjugates, complete the coupling and quench appropriately before introducing any stabilizer/diluent that may contain nucleophiles or thiols (general note; composition of this item is not specified).

Target Specificity

No antigen or target specificity applies. HRP‑StabilPLUS is a stabilizing reagent for HRP enzyme conjugates, not an antibody or affinity reagent.

• Clone/Isotype/Species reactivity: Not applicable.
• Item‑specific details: Not specified for this item; refer to CoA/Spec Sheet if any additional notes are provided.