GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Accession #
Q544U0
Protein Tag
No tag
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml. Loaded Recombinant Mouse LEPR (C-10His) on HIS1K Biosensor, can bind Recombinant Mouse Leptin with an affinity constant of 0.196 nM as determined in BLI assay.
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic
Bioactivity
Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml. Loaded Recombinant Mouse LEPR (C-10His) on HIS1K Biosensor, can bind Recombinant Mouse Leptin with an affinity constant of 0.196 nM as determined in BLI assay.
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (12 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
combinant Mouse Leptin Protein (L329647) - Protein Bioactivity Immobilized Recombinant Mouse LEPR Protein at 5 μg/mL can bind Recombinant Mouse Leptin Protein (L329647). The ED50 for this effect is 1.16 ng/mL.
Recombinant Mouse Leptin Protein (L329647) - SDS-PAGE 2 μg/lane of Recombinant Mouse Leptin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reviews
Customer Reviews
Application Protocols
Only protocols and tested applications provided by the manufacturer may be listed.
Manufacturer Applications: Not specified for this item in the Product Data.
Tested applications (e.g., WB, IHC, IF, FC), recommended dilutions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet.
Researchers should develop and optimize protocols empirically, using literature as a starting point and validating conditions for their specific cell types and assay readouts.
Biological Roles
The following summarizes known biological roles of murine leptin (literature/general). These are provided for scientific context and do not imply any specific performance characteristics for this item.
Origin and structure
Leptin is a secreted adipokine encoded by the Lep gene and classed as a class I cytokine-like hormone with a four-helix bundle fold.
Receptor and signaling
Binds the leptin receptor (LepR/Ob-R), particularly the long signaling isoform Ob-Rb. Engagement activates JAK2 and downstream STAT3, PI3K/AKT, and MAPK pathways, influencing transcriptional programs in target cells.
Physiological roles (non-clinical, general)
Energy homeostasis: Communicates adipose energy reserves to the central nervous system, modulating neuroendocrine pathways that influence energy expenditure and feeding behavior in murine systems.
Immune modulation: Influences innate and adaptive immune cell function, including effects on T-cell polarization and macrophage cytokine profiles in model systems.
Developmental and reproductive biology: Interfaces with endocrine axes that can affect developmental and reproductive parameters in animal models.
Biochemical properties relevant to function
Monomeric bioactive form; proper folding and a conserved disulfide bond are critical for receptor binding and signaling.
pH/ionic strength near physiological conditions generally support activity; denaturation or aggregation reduces receptor engagement.
Use this background to design receptor-specific assays, define readouts (e.g., STAT3 phosphorylation), and choose appropriate positive/negative controls. For item-specific activity and assay conditions, consult the CoA/Spec Sheet.
Buffer Applications
Item-specific note
PBS Only: The product is supplied without carrier proteins in phosphate-buffered saline (per Product Data). Exact PBS composition (e.g., phosphate/NaCl concentrations, pH) is Not specified for this item; refer to CoA/Spec Sheet.
Practical guidance (literature/general)
Typical PBS: Commonly 10–20 mM phosphate, 137 mM NaCl, 2.7 mM KCl, pH ~7.2–7.4 (literature). Prepare with ultrapure water and sterilize by 0.22 µm filtration for cell-based assays.
Dilution buffers: For signaling assays, maintain physiological ionic strength and pH. HEPES-buffered saline can be used for CO2-independent incubations. Avoid chelators if they interfere with downstream readouts.
Adsorption management: Because the product is Carrier Free, use low-binding plastics; adding inert proteins is generally avoided unless validated for the assay, as they can confound readouts.
Compatibility: PBS is broadly compatible with receptor-binding and cell signaling assays; verify compatibility for any enzyme-mediated reactions where phosphate might inhibit activity.
Storage/dilution strategy
Prepare single-use aliquots in PBS at assay-ready concentrations to avoid repeated freeze–thaw.
Keep samples on ice during setup; equilibrate to the required temperature immediately prior to application to cells or receptors.
For exact formulation details of the supplied PBS and recommended working concentrations, consult the CoA/Spec Sheet.
Green Alternatives
While the concept of “green alternatives” commonly targets organic solvents and reagents, sustainability considerations also apply to recombinant proteins.
Item-specific aspects
Animal Free: Production without animal-derived materials reduces reliance on animal-origin components, aligning with ethical sourcing and reducing variability and contamination risks.
PBS Only: Aqueous formulation eliminates organic solvents in the supplied product.
Laboratory sustainability (literature/general)
Cold-chain optimization: Plan consolidated experiments to minimize shipping frequency on ice and reduce cold-pack waste.
Aliquoting: Prepare working aliquots to avoid repeated freeze–thaw, decreasing product loss and waste.
Buffer preparation: Use high-purity water and prepare PBS in-house when appropriate to reduce packaging; sterilize by filtration.
Comparison (general)
Carrier-containing formulations: Can reduce adsorption losses but introduce animal-derived components (e.g., BSA), complicating downstream assays and sustainability goals.
Recombinant, animal-free systems: Favor reproducibility and reduce animal use; ensure expression platforms and purification resins are selected for low environmental impact when possible.
Trade-offs: Carrier-free proteins may require more careful handling to prevent adsorption/aggregation. Balance sustainability with experimental robustness by validating minimal-additive conditions first.
Pharmaceutical Uses
This product is designated For research use only and is not intended for therapeutic, diagnostic, or in vivo use.
Item-specific note
No pharmacopeial status, excipient role, or formulation directives are provided. Any clinical or manufacturing applications are out of scope for this research reagent.
General context (literature/general)
Recombinant cytokine-like proteins such as leptin are used in research to model receptor-mediated signaling, evaluate drug candidates targeting the leptin axis, and develop analytical assays (e.g., ELISA standards). These are preclinical research activities and do not imply suitability for pharmaceutical use.
Practical guidance for researchers
If the protein is to be used as a system-suitability or calibration material for analytical methods, establish traceability to internal standards and validate linearity and recovery within your specific matrix.
For any GLP/GMP-adjacent workflows, obtain and review detailed quality attributes (purity, identity, endotoxin) from the CoA/Spec Sheet and verify they meet your internal acceptance criteria.
No medical or clinical claims are made or implied for this product.
Physical Properties
Item-specific physicochemical specifications are limited in the provided data.
Provided for this item
Formulation note: PBS Only (no carrier proteins; per Product Data). Detailed excipient concentrations are Not specified for this item; refer to CoA/Spec Sheet.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Typical properties (literature/general for leptin protein)
Approximate molecular mass (monomer): ~16 kDa by sequence; may migrate ~16–18 kDa by SDS-PAGE depending on conditions (literature).
Isoelectric point (pI): Reported values for mouse leptin typically in the ~5–6 range, depending on sequence and calculation method (literature/computed).
Solubility: Soluble in physiological buffers (e.g., PBS) over a range of 0.01–1 mg/mL; aggregation can occur at higher concentrations or suboptimal pH/ionic strength (literature/general). Actual solubility limit for this preparation is Not specified for this item; refer to CoA/Spec Sheet.
Stability: Sensitive to repeated freeze–thaw; stability enhanced by avoiding surfactants and vigorous agitation (literature/general).
Not applicable to proteins
Boiling point, melting point, density, refractive index, SMILES/InChI: Not applicable to a recombinant protein and Not specified for this item; refer to CoA/Spec Sheet if needed.
Always consult the CoA/Spec Sheet for lot-specific concentration, buffer composition, and any stabilizers (none implied beyond “PBS Only”).
Quality & Grades
This product carries several quality designations. The following clarifies their implications for experimental planning.
Item-specific grades (per Product Data)
Animal Free: Manufactured without animal-derived components. Reduces risk of adventitious agents and improves consistency for cell-based assays and bioactivity studies.
Carrier Free: No carrier proteins (e.g., BSA, HSA, gelatin) added. Advantages: precise mass balance, avoids carrier-related background in binding/ELISA/cell assays; Consideration: increased adsorption risk to plastics—use low-bind tubes, add inert protein only if compatible with your assay.
Bioactive: Functional activity verified by the manufacturer using an appropriate assay (assay details not provided here). Facilitates signaling and receptor-binding experiments; nonetheless, verify activity under your specific conditions.
ActiBioPure™: Proprietary purity/activity designation by the manufacturer indicating high purity and activity; exact criteria Not specified for this item; refer to CoA/Spec Sheet.
PBS Only: Supplied in phosphate-buffered saline without additional stabilizers or carriers. Buffer composition details Not specified for this item; refer to CoA/Spec Sheet.
What is not specified for this item
Endotoxin level, host cell proteins/DNA, purity by SDS-PAGE/HPLC, and exact activity units are Not specified; consult the CoA/Spec Sheet for lot-specific metrics.
Practical guidance
Because the product is Carrier Free and Bioactive, plan for adsorption control (low-bind plastics) and rapid, cold handling to preserve activity.
Reaction & Applications
This is a recombinant protein, not a small-molecule reagent; classical organic “reactions” are not applicable. The following focuses on research applications relevant to leptin biology (literature/general), while noting that manufacturer-validated applications are not provided.
Typical research applications (literature/general)
Cell signaling: Activation of leptin receptor (LepR) pathways (JAK2/STAT3, PI3K/AKT, MAPK) in responsive murine cell lines or primary cells to study downstream transcriptional responses.
Binding assays: Quantitative receptor-binding or competition assays using recombinant LepR extracellular domains or LepR-expressing cells.
Functional studies: Assess leptin’s effects on metabolic enzyme expression, cytokine crosstalk, or neuronal signaling in murine models ex vivo.
Calibration standards: Use as a standard in immunoassays (e.g., ELISA) for murine leptin quantitation, after verifying compatibility.
Practical considerations (general)
Dosing: Prepare serial dilutions in cold PBS or assay buffer. Because the product is Carrier Free, use low-bind plastics to minimize loss at low ng/mL concentrations.
Timing/temperature: Perform treatments on ice for binding steps; warm to 37°C for signaling responses as appropriate.
Controls: Include vehicle controls, heat-inactivated leptin (if compatible) or receptor antagonists/neutralizing antibodies to confirm specificity.
Manufacturer-provided specific application data are Not specified for this item; consult CoA/Spec Sheet and perform pilot optimization in your system.
Reaction Conditions
Classical chemical reaction conditions are not applicable to this recombinant protein. For biological assays involving leptin, the following are general literature-based considerations.
Assay conditions (literature/general)
Temperature: Receptor-binding steps often performed on ice or at 4°C; signaling readouts (e.g., STAT3 phosphorylation) typically at 37°C for 5–60 min depending on cell type.
Buffer: Physiological buffers (PBS or HEPES-buffered saline), pH ~7.2–7.4. Serum-free or low-serum conditions may reduce background but can affect receptor responsiveness.
Concentration range: Commonly in the 0.1–500 nM range for in vitro studies, depending on cell sensitivity and receptor density; exact effective concentrations are assay- and cell-type dependent.
Additives: Avoid carrier proteins unless validated; divalent cations are generally tolerated but verify for your system.
Controls and readouts
Include vehicle controls and, where feasible, receptor blockade (e.g., anti-LepR antibodies) to confirm specificity.
Readouts: pSTAT3 by Western blot/flow, reporter assays, qPCR of leptin-responsive genes.
These conditions are general guidance only and not item-specific specifications. Manufacturer-validated reaction/assay conditions are Not specified for this item; consult the CoA/Spec Sheet and optimize experimentally.
Safety & Handling
Regulatory and hazard information (item-specific)
GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to the product SDS for authoritative information.
Research Use: For research use only (per Product Data). Not for human or veterinary use.
General laboratory handling (proteins; literature/general)
PPE: Laboratory coat, nitrile gloves, and safety glasses recommended. Handle using aseptic technique to avoid microbial contamination when working at low concentrations.
Avoid mechanical stress: Do not vortex aggressively; mix by gentle inversion to minimize foaming and aggregation.
Freeze–thaw: Avoid repeated freezing and thawing (explicitly stated in Product Data). Aliquot upon first thaw to preserve bioactivity.
Incompatibilities: Avoid strong oxidants and extremes of pH that can denature or oxidize amino acid side chains (e.g., cysteine, methionine, tryptophan). Minimize exposure to elevated temperature and surface adsorption (use low-bind plastics when possible).
Bioactivity considerations: Because this product is labeled Bioactive, maintain cold chain and avoid prolonged room-temperature handling to retain functional conformation.
First-aid overview (general)
Inhalation/ingestion/skin/eye: Rinse affected area with water; seek medical attention if irritation persists. Provide SDS to responders.
Always defer to the SDS and institutional biosafety procedures for risk assessment, spill response, disposal, and transport.
Solvent Selection
Item-specific note
Formulation: PBS Only (per Product Data), indicating the protein is provided in an aqueous phosphate-buffered saline matrix. Organic solvent selection is not applicable to this product.
Practical handling (literature/general for proteins)
Dilution: Use sterile, pH-appropriate physiological buffers (e.g., PBS or HEPES-buffered saline) compatible with your bioassay. Avoid organic cosolvents that can denature proteins.
Adsorption mitigation: For very low concentrations, consider using low protein-binding plastics. Because this item is Carrier Free, avoid adding proteins unless validated for your assay.
Surfactants: Non-ionic surfactants (e.g., very low concentrations of polysorbates) can reduce adsorption but may interfere with some bioassays; validate experimentally.
When to choose PBS vs alternatives (literature/general)
PBS: Routine cell signaling and receptor-binding assays at neutral pH, ionic strength approximating physiological conditions.
HEPES-buffered saline: Enhanced pH stability in CO2-independent workflows or extended room-temperature manipulations.
Tris buffers: Useful in biochemical assays but can interact with certain enzymes or crosslinkers; verify compatibility.
For this specific item, buffer composition and concentration ranges are Not specified; refer to CoA/Spec Sheet. Maintain low temperatures during all dilutions to preserve bioactivity.
Storage & Reconstitution
Item-specific storage and shipping (per Product Data)
Storage Conditions: Store at -20°C.
Avoid repeated freezing and thawing: Aliquot upon first thaw to preserve bioactivity.
Shipped In: Ice chest + ice pads (cold packs) to maintain refrigerated temperature during transit.
Additional handling guidance (general)
Temperature control: Keep on ice during handling. For short-term use, storage at 2–8°C for limited periods may be feasible, but this is not specified—follow your internal stability studies and the CoA/Spec Sheet.
Light and agitation: Protect from excessive agitation and foaming; normal ambient light is usually acceptable for proteins, but minimize unnecessary exposure.
Reconstitution and concentration
Reconstitution instructions, initial concentration, and exact buffer composition are Not specified for this item; refer to CoA/Spec Sheet. If supplied lyophilized or concentrated, reconstitute/dilute using sterile PBS consistent with “PBS Only,” validating concentration by A280 or protein assay as appropriate.
Aliquoting tips (general)
Prepare single-use aliquots in low-bind tubes, label with lot/concentration/date, and freeze promptly at -20°C or colder as validated. Avoid multiple freeze–thaw cycles to maintain the product’s Bioactive designation.
Always consult the CoA/Spec Sheet and SDS for lot-specific instructions and stability information.
Structure & Identity
Recombinant Mouse Leptin is a recombinant form of the murine adipokine hormone leptin (gene: Lep), produced for research use.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
SMILES / InChIKey: Not applicable for proteins; not specified.
Structural description (literature/general)
Primary structure: Leptin is a secreted protein of approximately 146 amino acids after signal peptide removal (mouse, literature), with a characteristic four-helix bundle cytokine-like fold.
Disulfide connectivity: Contains a conserved intramolecular disulfide bond essential for correct folding and bioactivity (literature).
Oligomeric state: Predominantly monomeric in dilute neutral buffers; can exhibit weak concentration-dependent self-association (literature).
Receptor: Binds the long form of the leptin receptor (LepR/Ob-Rb), a class I cytokine receptor (literature).
2D/functional group features (literature/general)
Protein biopolymer featuring peptide backbone amide linkages, side-chain functional diversity (acidic, basic, polar, hydrophobic residues), and a cystine linkage. No small-molecule stereocenters; chirality arises from L-amino acids in the polypeptide chain.
Note: Exact sequence, expression host, and post-translational features for this specific lot are Not specified for this item; refer to CoA/Spec Sheet.
Synthetic Utility
Not typically applicable. This product is a recombinant protein reagent rather than a synthetic building block or small-molecule catalyst.
Guidance
For biochemical conjugations (literature/general), leptin can, in principle, be derivatized via lysine ε-amines (e.g., NHS esters) or cysteine thiols if accessible; however, such modifications can impair bioactivity by perturbing receptor-binding epitopes. Any conjugation strategy should be empirically validated and is not covered by item-specific specifications.
Item-specific reactivity or conjugation guidance is Not specified for this item; refer to CoA/Spec Sheet and perform pilot studies if modification is contemplated.
Target Specificity
Only information present in the Product Data may be listed in this section.
Item-specific target details, clone, isotype, species reactivity, and validated applications: Not specified for this item; refer to CoA/Spec Sheet.
General knowledge about targets (e.g., LepR for leptin) is intentionally omitted here to comply with the requirement that this section reflect only product-provided data.
Frequently Asked Questions
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does Animal Free mean?
Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 181030-10-4.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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