Pectinase from Aspergillus niger, CAS No.9032-75-1

CAS: 9032-75-1 Cat. No.: P128776 EC Number: 232-885-6 PubChem CID: 87574303

GRADE & PURITY EnzymoPure™, ≥20 units/mg dry weight

Overview

Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. When used with Worthington purified cellulase, purified pectinase has been found to be highly successful for generating good yields of viable protoplasts in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. In general, a concentration range of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase) used at 24°C to 37°C for periods of 1 to 16 hours will yield good results.

Specifications

Product Name

Pectinase from Aspergillus niger, CAS No.9032-75-1

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥20 units/mg dry weight

CAS

9032-75-1

Enzyme Commission Number

4.2.2.10

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥20 units/mg dry weight

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

One Unit releases 1µmole of D-galacturonic acid from polygalacturonic acid per minute at 37°C, pH 5.0.