Phosphatase, Alkaline, CAS No.9001-78-9

CAS: 9001-78-9 Cat. No.: P128629 EC Number: 232-631-4 PubChem CID: 18985873

GRADE & PURITY Native, EnzymoPure™, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Accession #

P00634

Bioactivity

≥30 units/mg protein (25°C, pH 8.0)

Overview

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.

Unit Definitons

E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

Specifications

Product Name

Phosphatase, Alkaline, CAS No.9001-78-9

Synonyms

Alkaline phosphatase | Phosphatase, alkaline | ENTEROSTATIN,HUMAN | alkaline phosphomonoesterase | glycerophosphatase | phosphomonoesterase | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) | alkaline phosphatase | Apase, Escherichia coli |

Grade

Native, EnzymoPure™

Specifications & Purity

EnzymoPure™, Nature, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Biochemical and Physiological Mechanisms

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad

Bioactivity

≥30 units/mg protein (25°C, pH 8.0)

Accession #

P00634

CAS

9001-78-9

Enzyme Commission Number

I.U.B.: 3.1.3.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.