PIPES(PIPES) - UltraBio™, Ultra pure, ≥99%(T) , CAS No.5625-37-6

CAS: 5625-37-6 Cat. No.: P432927 Formula: C₈H₁₈N₂O₆S₂ Molecular Weight: 302.37 Beilstein Registry Number: 817713 EC Number: 227-057-6

GRADE & PURITY UltraBio™, Ultra pure, ≥99%(T)

Synonyms

FT-0635789 | G502H79V6L | NCGC00164485-01 | 2,2'-piperazine-1,4-diylbisethanesulfonic acid | 2-[(2-aminoacetyl)-methylamino]acetic acid | NSC157117 | NSC-157117 | 2,2'-(Piperazine-1,4-diyl)diethanesulfonicacid | CHEBI:44933 | AS-12650 | 3,9-bis(2,4-ditert

Storage

Room temperature

Overview

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples. , PIPES has been utilized in protein crystallization. , The use of PIPES in the reconstitution of dissociated tubulin α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8. A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli .

Specifications

Synonyms

FT-0635789 | G502H79V6L | NCGC00164485-01 | 2,2'-piperazine-1,4-diylbisethanesulfonic acid | 2-[(2-aminoacetyl)-methylamino]acetic acid | NSC157117 | NSC-157117 | 2,2'-(Piperazine-1,4-diyl)diethanesulfonicacid | CHEBI:44933 | AS-12650 | 3,9-bis(2,4-ditert

Specifications & Purity

UltraBio™, Ultra pure, ≥99%(T)

Storage

Room temperature

Grade

Ultra pure, UltraBio™

Purity

≥99%(T)

Names and Identifiers

Canonical Smiles

C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

IUPAC Name

2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid

InChIKey

IHPYMWDTONKSCO-UHFFFAOYSA-N

INCHI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

Isomeric SMILES

C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

WGK Germany

3

Molecular Weight

302.37

Beilstein

817713

Reaxy-Rn

817713

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=817713&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Diazinanes
      • Subclass Piperazines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Diazinanes
• Subclass: Piperazines
• Intermediate Tree Nodes: Not available
• Direct Parent: N-alkylpiperazines
• Alternative Parents: Sulfonyls Organosulfonic acids Alkanesulfonic acids Trialkylamines Azacyclic compounds Organopnictogen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aliphatic heteromonocyclic compounds
• Substituents: N-alkylpiperazine - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Alkanesulfonic acid - Tertiary amine - Tertiary aliphatic amine - Azacycle - Amine - Organic oxide - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Organosulfur compound - Organonitrogen compound - Hydrocarbon derivative - Aliphatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as n-alkylpiperazines. These are organic compounds containing a piperazine ring where the nitrogen ring atom carries an alkyl group.
• External Descriptors: PIPES

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: 1 M NaOH: 0.5M at20°C, clear, colorless
• Melt Point(°C): ＞300°C
• Molecular Weight: 302.400 g/mol
• XLogP3: -6.700
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 6
• Exact Mass: 302.061 Da
• Monoisotopic Mass: 302.061 Da
• Topological Polar Surface Area: 132.000 Ų
• Heavy Atom Count: 18
• Formal Charge: 0
• Complexity: 397.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1