Pronase, from Streptomyces griseus, CAS No.9036-06-0

CAS: 9036-06-0 Cat. No.: P736615 EC Number: 232-909-5

GRADE & PURITY EnzymoPure™, ~6000 units/g protein

Bioactivity

≥6000 U/g protein

Overview

Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids.

Application:

Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin，and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.

Specifications

Product Name

Pronase, from Streptomyces griseus, CAS No.9036-06-0

Synonyms

Actinase E | Pronase E | non-specific protease

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ~6000 units/g protein

Biochemical and Physiological Mechanisms

Pronase has wide-ranging specificity. Therefore, it is commonly used when extensive or complete degradation of proteins is required. Its applications include protein analysis in cell organelles, removal of proteins during DNA and RNA isolation, and the pr

Bioactivity

≥6000 U/g protein

CAS

9036-06-0

Enzyme Commission Number

3.4.24.4

Molecule Type

Enzyme

Storage and Shipping

Concentration

~6000 units/g protein

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

The amount of tyrosine per minute. For units designated as“U,” the amount of tyrosine was 1 μmol/min, and equal amounts of casein were extracted from casein at a temperature of 40 ° C and a pH of 7.5

References

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