Pyruvate oxidase, CAS No.9001-96-1

CAS: 9001-96-1 Cat. No.: P774057 EC Number: 232-644-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥15 U/mg enzyme powder; ≥50 U/mg protein

Accession #

P37063

Bioactivity

≥15 U/mg enzyme powder; ≥50 U/mg protein

Overview

Source: Microorganism 
Isoelectric point: 5.0 
Michaelis constant: 5.3×10^-4 M (Pyruvate) 
Optimum pH: 7.0-7.5                     Fig. 1
Optimum temperature: 40℃~50℃                     Fig. 3
pH Stability: 6.0~7.0 (37℃, 60min)                     Fig. 2
Thermal stability: <45℃ (pH 6.5, 30min)                     Fig. 4
Inhibitors: Cu2+, Ni2+, Zn2+, NaN3, Proclin, SDS, Triton X-100 
Effect of various chemicals:                     Table 1
Reaction:

Reaction:
  

Specifications

Product Name

Pyruvate oxidase, CAS No.9001-96-1

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),≥15 U/mg enzyme powder; ≥50 U/mg protein

Bioactivity

≥15 U/mg enzyme powder; ≥50 U/mg protein

Accession #

P37063

Predicted molecular weight

68 kDa (SDS-PAGE)

CAS

9001-96-1

Enzyme Commission Number

EC 1.2.3.3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥15 U/mg enzyme powder; ≥50 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (5 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will convert one micromole of pyruvate to acetylphosphate per min at pH 6.7at 37℃.

Frequently Asked Questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥90% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C, protected from light, desiccated. Avoid repeated freeze–thaw cycles. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9001-96-1.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.