Recombinant Alcohol Dehydrogenase (ADH), CAS No.9031-72-5

CAS: 9031-72-5 Cat. No.: rp226533 EC Number: 232-870-4 PubChem CID: 78120390

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥100 U/mg protein

Accession #

P00330,P00331,P07246,P10127,P38113

Expression system

E. coli

Bioactivity

≥100 U/mg protein

Overview

Source: Microorganism 
Isoelectric point: 6.5 
Michaelis constant: 8.0×10⁴M (Ethanol) 
Optimum pH: 9.5~10.5 Fig. 1
Optimum temperature: 50℃~60℃ Fig. 3
pH Stability: 6.0~9.5 (25℃, 20hr) Fig. 2
Thermal stability: <55℃ (pH 7.5, 30min) Fig. 4
Inhibitors: Cu2+, Ni2+, Zn2+, NEM, Proclin, Na-cholate, SDS, Triton X-100, Tween20  

Effect of various chemicals: Table 1

Reaction:

Specifications

Product Name

Recombinant Alcohol Dehydrogenase (ADH), CAS No.9031-72-5

Synonyms

aldehyde reductase | Alcohol:NAD+ oxidoreductase | ADH1 | ADH2 | ADH3 | ADH4 | ADH5 | ADC1 | ADR2 | NRC465 | ZRG5

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥100 U/mg protein

Bioactivity

≥100 U/mg protein

Accession #

P00330, P00331, P07246, P10127, P38113

Predicted molecular weight

40 kDa (SDS-PAGE)

CAS

9031-72-5

Enzyme Commission Number

EC 1.1.1.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥100 U/mg protein

Reconstitution

Reconstitute in sterile water to a concentration of less than 5.0 mg/mL.

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ (2 years). Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will convert one micromole of ethanol to acetaldehyde per min at pH8.8 at 25℃.