GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.7 mg/mL
ExactAb™, Validated, Recombinant, 0.7 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Entactin/NID Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 - 1/1000 IHC: 1/50-1/100 Protein Function Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. Post-translational N- and O-glycosylated.
Specifications
Product Name
Recombinant Entactin/NID Antibody - Primary antibody, specific to NID1, Rabbit IgG
Recombinant protein of human Entactin/NID (AA 844-1245).
Positive Control
WB: HeLa, A549, U251, U87-MG lysates. IHC: Human colon cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.7 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Entactin/NID Antibody (Ab101762) - Western Blot All lanes: Recombinant Entactin/NID Antibody (Ab101762) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 136 kDa Observed band size: 52, 93, 144 kDa The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:3084254,12215539, 22952693). Exposure time:66.5 s
Recombinant Entactin/NID Antibody (Ab101762) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Entactin/NID Antibody (Ab101762).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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