Recombinant Human Histone H3 Protein, >90% (SDS-PAGE)

Cat. No.: rp156635

GRADE & PURITY Carrier Free, Azide Free, His Tag, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

P68431

Protein Tag

N-His

Expression system

E. coli

Overview

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description：

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 histone family member E pseudogene, H3 histone family, member A, H3/A, H31_HUMAN, H3F3, H3FA, Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, HIST3H3, histone 1, H3a, Histone cluster 1, H3a

Specifications

Product Name

Recombinant Human Histone H3 Protein, >90% (SDS-PAGE)

Synonyms

Histone H3/a | Histone H3/b | Histone H3/c | Histone H3/d | Histone H3/f | Histone H3/h | Histone H3/i | Histone H3/j | Histone H3/k | Histone H3/l

Grade

Azide Free, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Azide Free, His-Tag, ≥90%(SDS-PAGE)

Purity

>90% (SDS-PAGE)

Expression System

E. coli

Amino Acids

1-136 aa

Sequence

MGHHHHHHMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Protein Tag

N-His

Accession #

P68431

Predicted molecular weight

15.4 kDa

SDS-PAGE

16.2 kDa, reducing conditions; 16.2 kDa, non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human Histone H3 Protein (rp156635)

Recombinant Human Histone H3 Protein (rp156635) - SDS-PAGE
Recombinant Human Histone H3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.4 kDa under reducing conditions, showing two bands at 16.2 kDa and 32.0 kDa under non-reducing conditions .