GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.3 mg/mL
ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human NELFe Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/50-1/100 Protein Function Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
Specifications
Product Name
Recombinant NELFe Antibody - Primary antibody, specific to NELFE, Rabbit IgG
Synonyms
Major histocompatibility complex gene RD | Negative elongation factor E | Negative elongation factor polypeptide E | NELF e | NELF-E | Nelfe | NELFE_HUMAN | Nuclear protein | RD | RD protein | RDBP | RD RNA binding protein | RDP | RNA-binding protein RD |
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species
Rabbit
Specificity
NELFE
Immunogen
Recombinant protein of human NELFe (AA 1-150)
Positive Control
WB: HeLa and NIH/3T3 cell lysates. IHC: Human breast cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.3 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant NELFe Antibody (Ab117537) - Western Blot All lanes: Recombinant NELFe Antibody (Ab117537) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 43 kDa Observed band size: 45, 47 kDa Exposure time: 2.3 s
Recombinant NELFe Antibody (Ab117537) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant NELFe Antibody (Ab117537). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Application
Application
Dilution info
WB
1/500-1/2000
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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