BioReagent, 50% v/v BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
This product utilizes recombinant Protein G as the ligand and a novel high-rigidity agarose bead as the matrix. Protein G specifically binds to the Fc region of antibodies, making this resin suitable for the purification of both monoclonal and polyclonal antibodies. High-purity antibodies can be obtained in a single step from animal ascites, serum, or cell culture supernatants. Due to its additional binding capability via the Fab region, Protein G affinity resin is more suitable for isolating immune complexes. It offers high binding capacity, excellent physicochemical stability, minimal ligand leakage, and a long service life.
Compared to Protein A, Protein G exhibits distinct IgG binding characteristics, particularly a stronger affinity for polyclonal antibodies.
Aladdin rProtein G Agarose Resin is stored in 20% ethanol, with a settled gel to storage solution ratio of 1:1. The product specification refers to the actual volume of the settled gel.
| Parameter | Value |
| Matrix | High-rigidity agarose |
| Ligand | Recombinant Protein G |
| Average Particle Size | 75 μm |
| Ligand Density | 6–10 mg Protein G/mL resin |
| Dynamic Binding Capacity | ≥35 mg human IgG/mL resin |
| Maximum Flow Rate (25°C) | 300 cm/h |
| Recommended Flow Rate | <150 cm/h |
| Working pH Range | 3-9 |
| Cleaning-in-Place (CIP) pH Range | 2-10 |
Protocol
Due to the varying affinity of the resin for antibodies from different species and subtypes, and the differing buffer conditions required to maintain antibody activity, users are advised to optimize binding and elution conditions.
A reference purification procedure is provided below:
1.Equilibration: Equilibrate the column with 10 column volumes (CV) of Buffer A (20 mM phosphate buffer + 0.15 M NaCl, pH 7.0) until the baseline stabilizes.
2.Loading: Load the sample. For solid samples, dissolve in Buffer A. For liquid samples, dialyze against Buffer A prior to loading.
3.Washing: Wash the column with 5 CV of Buffer A until the UV baseline returns to baseline levels.
4.Elution: Elute bound antibodies using a linear gradient over 10 CV to 100% Buffer B (e.g., 20 mM citrate, pH 3.0; 0.1 M glycine, pH 3.0; or 20 mM sodium acetate, pH 3.0).
5.Neutralization: Collect elution fractions and immediately neutralize using an alkaline buffer (e.g., 1.0 M Tris-HCl, pH 9.0, or other suitable buffer) to adjust the antibody solution to a stable pH.
6.Regeneration & Cleaning-in-Place (CIP): After multiple cycles, regenerate the column using one of the following methods:
Option 1: Wash with 3–5 CV of 0.1 M acetic acid or 0.1 M acetic acid/20% ethanol. Rinse with buffer until neutral pH is reached before reuse.
Option 2: Wash with 3–5 CV of 70% ethanol or 6 M guanidine hydrochloride. Rinse with 3–10 CV of purified water. Equilibrate with buffer to neutral pH before reuse.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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