Sarcosine oxidase (SOX), CAS No.9029-22-5

CAS: 9029-22-5 Cat. No.: rp216177 EC Number: 232-850-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥15 U/mg enzyme powder; expressed in E.coli

Accession #

P40859

Expression system

E. coli

Bioactivity

≥15 U/mg enzyme powder

Overview

Application
Used in the development and mass formulation of enzymatic creatinine reagents.
Enzymatic properties (note that -3, 2+ are superscripts)
Source: Microorganism
Enzyme Committee Number: EC 1.5.3.1
Molecular weight: 45 kDa (SDS-PAGE)
Isoelectric point: 5.3
Km value: 5.6×10⁻³M(Sarcosine)
Inhibitors: SDS, Zn²⁺, Cd²⁺
Optimal pH: 8.0                                                          Figure 1
Optimum temperature: 50℃                                      Figure 2
pH stability: 7.0-9.5 (25℃,16h)                                  Figure 3
Thermal stability: Stable below 50°C (pH7.5,30min) Figure 4
Stability: -25 ~ -15℃ standing store for 12 months
More than 90% activity                                              Figure 5

Assay method for activity
1. Principle

The resulting Quinoneiminedye can be detected at 555nm.

2. Definition of enzyme activity
Unit enzyme activity is defined as the amount of enzyme required to catalyze the production of 1 μmol H2O2 per minute under the following conditions.

3. Reagent preparation
Reagent I: 0.2M Tris-HCl buffer, pH8.0.
Reagent II: 1.0M sarcosine solution (8.9g sarcosine dissolved in 100mLUP water).
Reagent III: 1kU/mL POD.
Reagent IV: 50 mm4-AA solution (1.016g4-AA dissolved in 100mLUP water).
Reagent V: 50mM TOOS solution (1.657gTOOS dissolved in 100mLUP water).
Enzyme diluent: 20mM Tris-HCl, pH8.0, 2mM EDTA.
Prepare the reaction mixture as follows:

Reagent I   5mL
Reagent II 10mL
Reagent III 0.25mL
Reagent IV 1.5mL
Reagent V 1.5mL double steaming water 31.75mL
A total of 50mL can be used for 50 reactions.

4. Operation procedure
1. Add 1 ml reaction mixture to 1mL colorimetric dish.
2.37°C temperature bath for 5min.
3. Add 20μL of enzyme liquid to the reaction mixture and mix well.
4.37°C reaction, detection of sample absorbance change within 1min at 555nm (∆As)
* Replace enzyme liquid with enzyme diluent, other steps are the same, the absorbance of the resulting solution is blank absorbance (∆Ab)
∆A=∆As-∆Ab

5. Vitality computing

1.020: total volume of reaction liquid (mL);
0.020: enzyme liquid volume (mL);
t: Reaction time (1min);
df: dilution ratio;
1/2:1 mole of hydrogen peroxide to generate 1/2 mole of quinone imide dye;
1.0: optical path length (cm);
C: Enzyme concentration (mg/mL);
39.2: Under standard reaction conditions, the millimolar absorption coefficient of the color group at 555nm (cm2/μmol).

Specifications

Product Name

Sarcosine oxidase (SOX), CAS No.9029-22-5

Synonyms

oxygen oxidoreductase (demethylating) | sarcosine oxidasee (formaldehyde-forming) | monomeric sarcosine oxidase | MSOX | sarcosine oxidasee | SAO | soxA

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥15 U/mg enzyme powder; expressed in E.coli

Biochemical and Physiological Mechanisms

Catalyzes the oxidative demethylation of sarcosine. Can also oxidize other secondary amino acids such as N-methyl-L-alanine.

Bioactivity

≥15 U/mg enzyme powder

Accession #

P40859

Predicted molecular weight

45 kDa

CAS

9029-22-5

Enzyme Commission Number

EC 1.5.3.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥15 U/mg enzyme powder; expressed in E.coli

Reconstitution

Reconstitute in water.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (36 months). Upon reconstitution, it is recommended to aliquot.

Unit definition

The amount of enzyme that catalyzes the formation of 1 μmol H₂O₂ per minute under the specified conditions.

References

1. Ziyi Tong, Shengyan Hou, Zhenkun Zhang, Zhen Liu, Yifei Zhang.  (2024)  Biochemical approaches for decoding the information stored with metabolites.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.136618]

2. Yan Tao, Chongying Liao, Fangli He, Yixuan He, Sihui Yang, Liu-Pan Yang, Li-Li Wang.  (2025)  Smartphone-assisted colorimetric sensing platform based on Fe-LCDs nanozymes for detection of creatine in meat.  FOOD CHEMISTRY,      [PMID:40381557] [10.1016/j.foodchem.2025.144754]

3. Fangbing Wang, Qiwen Peng, Yongheng Zhang, Min Zhang, Guoyue Shi.  (2026)  Five-in-one colorimetric multiplexed point-of-care testing of blood biomarkers via Janus separation-sensing membrane.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2026.174104]