Sudan III Staining Solution - BioReagent, Biological Stain, for microscopy, 0.1% , CAS No.85-86-9

CAS: 85-86-9 Cat. No.: S774204 Formula: C₂₂H₁₆N₄O Molecular Weight: 352.39 Beilstein Registry Number: 931185 EC Number: 201-638-4 PubChem CID: 62331

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.1%

Storage

Protected from light,Room temperature

Shipped In

Normal

Overview

Sudan dye is more soluble in lipids than in solvents. When the dye contacts lipid-containing samples, a large amount of dye penetrates into lipid structures and stains them red.

Instructions for Use

1. Rinse coverslips 3 times with Hanks solution.

2. Fix samples with formaldehyde-calcium fixative for 20 minutes.

3. Rinse coverslips 3 times with distilled water.

4. Wash coverslips 3 times with 95% ethanol.

5. Fully cover specimens with Sudan III staining solution and stain for 10-30 minutes.

6. Rinse coverslips 3 times with 95% ethanol.

7. Mount slides with glycerol gelatin and observe.

Staining Result:

Lipids

Orange-red

Precautions:

1. Prepare formaldehyde-calcium fixative personally: mix 10 mL formaldehyde and 1 g calcium chloride, add water to make 100 mL solution.

2. Conduct preliminary tests with 1 to 2 samples before formal use.

3. Wear lab coat and gloves for personal protection during operation.

Specifications

Specifications & Purity

BioReagent, Biological Stain, for microscopy, 0.1%

Stability And Storage

Store at room temperature for 12 months. Store in the dark.

Storage

Protected from light,Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy

Names and Identifiers

Canonical Smiles

C1=CC=C(C=C1)N=NC2=CC=C(C=C2)N=NC3=C(C=CC4=CC=CC=C43)O

IUPAC Name

1-[(4-phenyldiazenylphenyl)diazenyl]naphthalen-2-ol

InChIKey

FHNINJWBTRXEBC-UHFFFAOYSA-N

INCHI

1S/C22H16N4O/c27-21-15-10-16-6-4-5-9-20(16)22(21)26-25-19-13-11-18(12-14-19)24-23-17-7-2-1-3-8-17/h1-15,27H

Isomeric SMILES

C1=CC=C(C=C1)N=NC2=CC=C(C=C2)N=NC3=C(C=CC4=CC=CC=C43)O

WGK Germany

3

RTECS

QK4250000

PubChem CID

62331

Molecular Weight

352.39

Beilstein

931185

Reaxy-Rn

6528173

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Azobenzenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Azobenzenes
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Azobenzenes
• Alternative Parents: Naphthols and derivatives 1-hydroxy-2-unsubstituted benzenoids Benzene and substituted derivatives Azo compounds Propargyl-type 1,3-dipolar organic compounds Organopnictogen compounds Organooxygen compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic homopolycyclic compounds
• Substituents: Azobenzene - 2-naphthol - Naphthalene - 1-hydroxy-2-unsubstituted benzenoid - Monocyclic benzene moiety - Benzenoid - Azo compound - Propargyl-type 1,3-dipolar organic compound - Organic 1,3-dipolar compound - Organopnictogen compound - Organooxygen compound - Organonitrogen compound - Organic oxygen compound - Organic nitrogen compound - Hydrocarbon derivative - Aromatic homopolycyclic compound
• Description: This compound belongs to the class of organic compounds known as azobenzenes. These are organonitrogen aromatic compounds that contain a central azo group, where each nitrogen atom is conjugated to a benzene ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Sensitivity: Light Sensitive
• Melt Point(°C): 199°C

Frequently Asked Questions

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 85-86-9, the molecular formula is C22H16N4O, and the molecular weight is 352.39 g/mol. InChIKey FHNINJWBTRXEBC-UHFFFAOYSA-N.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.