2,4-Dinitrophenylhydrazine(DNPH) - AR, ≥98%(HPLC) , CAS No.119-26-6

CAS: 119-26-6 Cat. No.: D112074 Molecular Weight: 198.14 Beilstein Registry Number: 615586 EC Number: 204-309-3
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GRADE & PURITY AR ? Analytical Reagent grade — high-purity chemicals meeting strict assay limits for lab analysis. Use when accuracy matters and trace impurities could skew results. ≥98%(HPLC)
Synonyms
2,4-dinitrophenyl hydrazine | CHEBI:66932 | Dnp-hydrazine | BB 0240515 | NSC 5709 | DTXSID2059485 | NSC5709 | NSC-5709 | STK365303 | F0722-8833 | (2,4-dinitrophenyl)-hydrazine
Storage
Protected from light,Room temperature,Argon charged
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Qty
5g
D112074-5g
10
$19.90
25g
D112074-25g
≥10

$57.90

$67.90
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Why this grade

AR, ≥98%(HPLC) AR for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Protected from light,Room temperature,Argon charged Ships FedEx DG Service Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 138 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis.The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).
2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.


Application:

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls.It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

Specifications

Synonyms
2, 4-dinitrophenyl hydrazine | CHEBI:66932 | Dnp-hydrazine | BB 0240515 | NSC 5709 | DTXSID2059485 | NSC5709 | NSC-5709 | STK365303 | F0722-8833 | (2, 4-dinitrophenyl)-hydrazine
Specifications & Purity
AR, ≥98%(HPLC)
Storage
Protected from light, Room temperature, Argon charged
Shipped In
FedEx DG Service
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
AR
Purity
≥98%(HPLC)
Names and Identifiers
Canonical SmilesC1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])NN
IUPAC Name(2,4-dinitrophenyl)hydrazine
InChIKeyHORQAOAYAYGIBM-UHFFFAOYSA-N
INCHI1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2
Isomeric SMILES C1=CC(=C(C=C1[N+](=O)[O-])[N+](=O)[O-])NN
UN Number 3380
Packing Group I
Molecular Weight 198.14
Beilstein 615586
Reaxy-Rn 615586
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=615586&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

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✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

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📊 Datasheet

Quick-reference summary of product specifications and applications.

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🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

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Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassBenzenoids
ClassBenzene and substituted derivatives
SubclassNitrobenzenes
Intermediate Tree Nodes Not available
Direct ParentNitrobenzenes
Alternative Parents Phenylhydrazines  Nitroaromatic compounds  Propargyl-type 1,3-dipolar organic compounds  Organic oxoazanium compounds  Organopnictogen compounds  Organic zwitterions  Organic oxides  Hydrocarbon derivatives  Hydrazines and derivatives  
Molecular FrameworkAromatic homomonocyclic compounds
Substituents Nitrobenzene - Nitroaromatic compound - Phenylhydrazine - C-nitro compound - Organic nitro compound - Organic oxoazanium - Allyl-type 1,3-dipolar organic compound - Propargyl-type 1,3-dipolar organic compound - Organic 1,3-dipolar compound - Hydrazine derivative - Organic zwitterion - Organopnictogen compound - Organonitrogen compound - Organic nitrogen compound - Organic oxygen compound - Organic oxide - Hydrocarbon derivative - Aromatic homomonocyclic compound
DescriptionThis compound belongs to the class of organic compounds known as nitrobenzenes. These are compounds containing a nitrobenzene moiety, which consists of a benzene ring with a carbon bearing a nitro group.
External Descriptors a small molecule
3D Structure
Interactive Chemical Structure Model





Associated Targets(Human)
MPO Tchem Myeloperoxidase (1002 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

47 results found

Lot NumberCertificate TypeDateItem
D2615287Certificate of AnalysisMar 09, 2026 D112074
D2615283Certificate of AnalysisMar 09, 2026 D112074
C2618515Certificate of AnalysisMar 09, 2026 D112074
C2618362Certificate of AnalysisMar 09, 2026 D112074
L2522075Certificate of AnalysisJan 04, 2026 D112074
I2524271Certificate of AnalysisMar 05, 2025 D112074
I2524286Certificate of AnalysisMar 05, 2025 D112074
C2513838Certificate of AnalysisMar 04, 2025 D112074
F2530217Certificate of AnalysisMar 04, 2025 D112074
F2530010Certificate of AnalysisMar 04, 2025 D112074
C2513839Certificate of AnalysisMar 04, 2025 D112074
B2517017Certificate of AnalysisJan 22, 2025 D112074
L2409333Certificate of AnalysisNov 19, 2024 D112074
K2427655Certificate of AnalysisNov 18, 2024 D112074
K2427400Certificate of AnalysisNov 18, 2024 D112074
K2427399Certificate of AnalysisNov 18, 2024 D112074
H2412403Certificate of AnalysisJul 30, 2024 D112074
H2412402Certificate of AnalysisJul 30, 2024 D112074
J2412031Certificate of AnalysisJul 30, 2024 D112074
F2429218Certificate of AnalysisJun 20, 2024 D112074
F2429240Certificate of AnalysisJun 20, 2024 D112074
F2411365Certificate of AnalysisApr 26, 2024 D112074
D2407214Certificate of AnalysisMar 26, 2024 D112074
D2407212Certificate of AnalysisMar 26, 2024 D112074
L2318271Certificate of AnalysisDec 12, 2023 D112074
L2318270Certificate of AnalysisDec 12, 2023 D112074
J2331139Certificate of AnalysisOct 20, 2023 D112074
J2331137Certificate of AnalysisOct 20, 2023 D112074
F2310104Certificate of AnalysisApr 19, 2023 D112074
F2310103Certificate of AnalysisApr 19, 2023 D112074
F2310099Certificate of AnalysisApr 19, 2023 D112074
B2310430Certificate of AnalysisNov 23, 2022 D112074
B2310431Certificate of AnalysisNov 23, 2022 D112074
B2310429Certificate of AnalysisNov 23, 2022 D112074
I2224211Certificate of AnalysisAug 16, 2022 D112074
I2224213Certificate of AnalysisAug 16, 2022 D112074
I2224209Certificate of AnalysisAug 16, 2022 D112074
I2224212Certificate of AnalysisAug 16, 2022 D112074
I2224201Certificate of AnalysisAug 16, 2022 D112074
H2206889Certificate of AnalysisJul 28, 2022 D112074
H2206893Certificate of AnalysisJul 28, 2022 D112074
H2206892Certificate of AnalysisJul 28, 2022 D112074
H2211003Certificate of AnalysisJul 28, 2022 D112074
H2211002Certificate of AnalysisJul 28, 2022 D112074
H2206891Certificate of AnalysisJul 28, 2022 D112074
H2206890Certificate of AnalysisJul 28, 2022 D112074
C2210028Certificate of AnalysisMar 19, 2022 D112074

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Chemical and Physical Properties
SensitivityAir and Light Sensitive
Flash Point(°C)14°C
Melt Point(°C)196~199°C
Molecular Weight198.140 g/mol
XLogP31.500
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count6
Rotatable Bond Count1
Exact Mass198.039 Da
Monoisotopic Mass198.039 Da
Topological Polar Surface Area130.000 Ų
Heavy Atom Count14
Formal Charge0
Complexity235.000
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count1
Documents & Articles
Citations of This Product
References
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