Alcian Blue Staining Solution (pH 1.0) - BioReagent, Biological Stain, for microscopy

Cat. No.: A774857

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Synonyms

Alcian Blue & Nuclear Fast Red Staining Kit, pH1.0 | Alnin Blue–Nuclear fast red Staining Kit

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Overview

  Alcian Blue (also known as Alcian blue) is a copper phthalocyanine conjugated dye originally used for textile fiber dyeing. This cationic dye binds to acidic groups, i.e., Alcian blue forms insoluble complexes with anionic groups in tissues such as carboxyl and sulfate groups. Alcian blue consists of a central copper‑containing phthalocyanine ring linked via thioether bonds to four isothiouronium groups. These isothiouronium groups are moderately alkaline, giving Alcian blue its cationic character. At pH 2.5, carboxyl groups in tissues ionize, carrying a negative charge, and form salt bonds with the cations in Alcian blue, thereby staining acidic mucus substances containing carboxyl groups (sulfated mucins and sialomucins).

  Alcian Blue Staining Solution (pH 1.0) utilizes different pH levels of the dye to distinguish classes of mucus substances. At pH 1.0, carboxyl groups (COOH) do not stain, while sulfate groups (OSO₃H) stain. At pH 2.5, carboxyl groups stain well but sulfated mucins stain poorly. At pH 1.0, sulfate groups in tissues ionize, carrying a negative charge, and form salt bonds with the cations in Alcian blue, staining tissues containing sulfate groups (e.g., sulfated mucus substances). Neutral mucins (such as neutral mucins in gastric mucosa and Brunner’s glands) do not react with Alcian blue.

A774857	Component	3×50 mL	3×100 mL	Storage
A774857A	Alcian Blue Acidification Solution	50 mL	100 mL	RT.
A774857B	Alcian Blue Staining Solution	50 mL	100 mL	RT. Store in the dark
A774857C	Nuclear Fast Red Staining Solution	50 mL	100 mL	RT. Store in the dark

Materials to Be Prepared by User

  Graded ethanol series, distilled water

Instructions for Use

1. Deparaffinize in xylene, rehydrate through a graded ethanol series, and rinse in distilled water.

2. Immerse in Alcian Blue Acidification Solution for 5 minutes.

3. Stain in Alcian Blue Staining Solution for 30 minutes. Rinse under running water.

4. Rinse briefly with Alcian Blue Acidification Solution.

5. Without water rinse, blot off excess Alcian Blue Acidification Solution using filter paper.

6. Counterstain in Nuclear Fast Red Staining Solution. Rinse under running water.

7. Dehydrate routinely through ethanol, clear in xylene, and mount with neutral balsam.

Precautions

1. Use 10% neutral formalin as fixative.

2. This method can distinguish sulfated mucins from proteoglycans; its pH = 1.0.

3. Opened reagents should be used within 6 months after opening. Tighten the cap immediately after each use to prevent evaporation or deterioration.

4. For your safety and health, please wear a lab coat and disposable gloves during operation.

5. This product is for research use only; any other use is strictly prohibited.

Staining Results

Tissue Component	Color
Sulfated mucins	Blue
Non‑sulfated acidic mucus substances	Unstained
Nuclei	Light red

Specifications

Synonyms

Alcian Blue & Nuclear Fast Red Staining Kit, pH1.0 | Alnin Blue–Nuclear fast red Staining Kit

Specifications & Purity

BioReagent, Biological Stain, for microscopy

Stability And Storage

Store at room temperature long term (6 months). Store in the dark.

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy

Names and Identifiers

• PH: 1.0

Chemical and Physical Properties

• Sensitivity: Light-sensitive