Amylase, CAS No.9000-90-2

CAS: 9000-90-2 Cat. No.: A757775 EC Number: 232-565-6

GRADE & PURITY EnzymoPure™, 480 KNU-B/g

Accession #

P00692

Bioactivity

≥480 KNU-B/g

Overview

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research.

Application：

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A7595 is from Bacillus amyloliquefaciens and is supplied as a liquid. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis.α-Amylase from Bacillus amyloliquefaciens has been used to hydrolyze sweetpotato amylopectin to identify cluster structure. The enzyme has been used to hydrolyze sago palm starch to reducing sugars, which are then used for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The enzyme catalyzes amylolysis of gelatinised waxy maize starch to produce reducing sugars. 

Biochemical mechanism：

α-Amylase is an endoamylase that randomly hydrolyzes the α-(1,4) glucan linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme works best at high temperatures, in the range of 70-90 ° Celcius.
The pH range for the α-amylase of B.amyloliquefaciens is 5.5 to 6.5, with a single maximum at 5.9. The activity is rapidly inactivated below pH 5.0.
An endoamylase that randomly hydrolyzes α-(1→4)-glycosidic linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme is active at high temperatures (70−90 °C).

Specifications

Product Name

Amylase, CAS No.9000-90-2

Synonyms

BAN® 480 L | 1,4-α-D-Glucan-glucanohydrolase | 1,4-alpha-D-Glucan-glucanohydrolase | alpha-amylase | glycogenase | mesotherm α-Amylase

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, 480 KNU-B/g

Biochemical and Physiological Mechanisms

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. Cofactor: Protein has several cofactor binding sites: Ca²⁺. Note: Binds 3 Ca²⁺ ions per subunit. Na⁺. Note: Binds 1 sod

Bioactivity

≥480 KNU-B/g

Accession #

P00692

CAS

9000-90-2

Enzyme Commission Number

3.2.1.1

Molecule Type

Enzyme

Legal Information

BAN is a trademark of Novozymes Corp.

Storage and Shipping

Concentration

480 KNU-B/g

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Unit definition

The activity of α-amylase is determined relative to a standard enzyme reference, and the enzyme activity unit is KNU(B)/g.