APTSTAT3-9R acetate - ≥95% , CAS No.A413941

CAS: A413941 Cat. No.: A413941 Formula: C₂₂₃H₃₃₀N₈₀O₅₁(free basis) Molecular Weight: 4947.51(free basis)

GRADE & PURITY ≥95%

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Specifications

Specifications & Purity

≥95%

Biochemical and Physiological Mechanisms

APTSTAT3-9R is a specific STAT3-binding peptide with addition of a cell-penetrating motif. The treatment of APTSTAT3-9R in various types of cancer cells blocks STAT3 phosphorylation and reduces expression of STAT targets.

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥95%

Names and Identifiers

Molecular Weight

4947.51(free basis)

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro Water: 100 mg/mL (20.21 mM)
• Sensitivity: Moisture sensitive；Light sensitive

Application Protocols

No vendor-validated application protocols are provided for this item. The following are general, literature-based starting points for peptide tools; adjust to your system.

• Cell treatment (general):
  1. Reconstitute to 1–10 mM stock in sterile water, dilute acid, or DMSO depending on solubility.
  2. Dilute into pre-warmed complete media to desired final concentration (e.g., 0.1–10 μM). Keep final DMSO ≤0.1–0.5% v/v.
  3. Incubate cells for 0.5–24 h. Include vehicle and peptide control groups.
  4. Harvest for endpoint assays (e.g., phospho-STAT3 Western blot, reporter activity, qPCR).
• Stability/adsorption checks:
  • Assess peptide stability in media over time by sampling and LC–MS or HPLC.
  • Use low-binding plasticware; add carrier protein (e.g., 0.1% BSA) if nonspecific adsorption is problematic.
• Sterility:
  • Filter reconstituted solution (0.22 μm) if sterility is required. Work aseptically.

These steps are provided as general guidance only. Item-specific instructions may differ and should be taken from the CoA/Spec Sheet if available.

Biological Roles

No item-specific biological mechanism is provided in the product data. The following is general, literature-based context relevant to peptide tools named for STAT3-related research.

• STAT3 pathway (general): Signal Transducer and Activator of Transcription 3 (STAT3) is a transcription factor activated by upstream kinases (e.g., JAKs), dimerizes upon Tyr705 phosphorylation, translocates to the nucleus, and regulates gene expression in diverse biological processes (development, immune signaling, metabolism). Temporal and spatial regulation is critical; dysregulated signaling has been widely studied in basic research.
• CPP-fused peptides (general): Short cationic sequences such as poly-arginine (e.g., 9R) can facilitate cellular uptake via endocytosis and direct translocation, enabling intracellular delivery of cargo peptides that modulate protein–protein interactions.
• Research utility:
  • Acute, reversible perturbation of STAT3-associated nodes to map signaling dependencies and feedback.
  • Tool compounds for target validation, pathway mapping, and phenotypic screening in cell culture.
• Considerations:
  • Uptake efficiency, endosomal escape, and proteolytic stability vary by cell type and media conditions.
  • Include appropriate controls to account for CPP-related effects on membranes and signaling.

No therapeutic or clinical claims are made. For precise sequence-specific roles, consult the CoA/Spec Sheet for this item.

Buffer Applications

This product is a peptide research tool; it is not a classical buffering agent. However, buffer choice is critical for handling and experiments.

• Reconstitution buffers (general guidance):
  • Start with water, PBS, or HEPES buffer (pH 6.8–7.4). If solubility is limited, use 0.1–1% acetic acid or 0.01–0.1 M HCl to initiate dissolution, then titrate to the desired pH with sterile base.
  • For cell work, ensure isotonicity and serum compatibility; sterile-filter through 0.22 μm when needed.
• Additives (general):
  • 1–5% serum albumin or low concentrations of non-ionic detergents can reduce adsorption to plastics; validate that these do not confound readouts.
• Storage buffers:
  • Aliquot into low-binding tubes; avoid phosphate when metal-catalyzed oxidation is a concern. Include antioxidants only if validated for your assay.
• Electrophoresis/sample prep:
  • For Western blot standards or pulldown controls, dissolve in standard Laemmli/SDS sample buffer as appropriate.

Item-specific buffer recommendations are not provided; optimize conditions empirically and consult the CoA/Spec Sheet for any provided solubility notes.

Green Alternatives

For peptide research tools, “green” considerations focus on solvent and workflow choices rather than alternative compounds.

• Solvent choices (general):
  • Prefer aqueous buffers over high fractions of organic solvents.
  • If an organic co-solvent is necessary, use minimal DMSO and dilute promptly into water-based media; avoid chlorinated solvents entirely for dissolution.
• Process considerations:
  • Prepare concentrated stocks to minimize repeated weighing and waste.
  • Use sterile, reusable glassware where compatible to reduce single-use plastics; balance with sterility requirements.
• Comparison (general): | Practice | Environmental impact | Trade-offs | |---|---|---| | Aqueous dissolution | Lower VOC emissions | May require mild acidification to achieve solubility | | DMSO stock + aqueous dilution | Moderate | Ensures solubility; manage DMSO exposure in cells | | Lyophilized single-use aliquots | Reduces freeze-thaw waste | More packaging material |
• Alternative modalities:
  • If pathway probing is the goal, consider genetic modulation (siRNA/CRISPR) or small-molecule tool compounds, each with distinct waste profiles and off-target risks.

These are general sustainability notes; no item-specific environmental metrics are provided.

Pharmaceutical Uses

• This product is supplied strictly for research use only (per product data). It is not intended for human or veterinary use, drug, household, or other applications.
• No pharmacopeial status, excipient role, or GMP-related information is provided for this item; do not use in manufacturing or clinical settings.
• Formulation context for research (general):
  • For in vitro studies, prepare sterile aqueous or DMSO stocks and dilute into cell culture media, monitoring final solvent content.
  • For exploratory in vivo research (if permitted by your institution and regulations), rigorous characterization of purity, endotoxin, and sterility is required; these specifications are not provided here and must be established independently.
• Stability considerations (general):
  • Peptide acetate salts may be sensitive to oxidation and hydrolysis; protect from light, store at −20°C under inert gas as specified for this item, and avoid repeated freeze–thaw cycles.

No claims are made regarding therapeutic benefit, dosage forms, or clinical utility.

Physical Properties

Item-specific physicochemical specifications are not provided in the product data.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility (general/literature guidance for peptide acetate salts):
  • Often soluble in water, aqueous buffers (e.g., PBS), or dilute acids (e.g., 0.1%–1% acetic acid) due to cationic residues; initial dissolution in sterile water or aqueous buffer is commonly effective.
  • If poorly soluble, prepare a small DMSO stock (e.g., 10–50 mM) then dilute into buffer; final DMSO ≤0.1–1% v/v for cell work (literature guidance).
• pH effects (general): cationic peptides typically show improved solubility at slightly acidic to neutral pH; aggregation can increase near isoelectric points.
• Hygroscopicity: peptide acetate salts can be hygroscopic; minimize exposure to ambient humidity.
• Thermal behavior: peptides generally decompose prior to boiling; melting point is seldom meaningful for lyophilized peptide solids.
• UV characteristics (general): peptide backbones and aromatic residues absorb in the 190–230 nm and 255–280 nm regions; quantify by weight or amino-acid analysis when extinction coefficient is unknown.
• Density, refractive index, logP, pKa: Not specified for this item; refer to CoA/Spec Sheet.

Quality & Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• What to expect for peptide tools (general guidance):
  • Peptide products are commonly supplied as lyophilized acetate salts with specified purity by HPLC (e.g., >90–98% area) and identity verified by MS. Since this listing does not state such values, verify on the CoA/Spec Sheet.
  • Counterion content (acetate) and residual solvents/water vary with lyophilization and desalting; confirm by CoA if critical for quantitative studies.
  • Endotoxin and bioburden are typically controlled for cell-based assays if labeled accordingly; absent such claims here, treat as a standard research-grade chemical.
• Stabilizers: Not specified for this item; refer to CoA/Spec Sheet. If present, stabilizers (e.g., mannitol, trehalose) can influence solubility and mass yield.
• Batch-to-batch consistency: For peptide reagents, review chromatograms and MS traces in the CoA; small shifts in counterion content or hydration can alter mass recovery on reconstitution.
• Documentation: Request analytical HPLC, MS, and, if available, amino-acid analysis for your lot if regulatory or quantitative needs require it.

Reaction & Applications

This item is a research-use peptide tool rather than a synthetic reagent; classical organic reaction applications (e.g., cross-coupling, Grignard) do not apply. The following focuses on biological research uses based on literature conventions for STAT-pathway-targeted, CPP-fused peptides. No item-specific activity claims are made here.

• Typical application domain (literature/general):
  • Cell biology and chemical biology studies interrogating STAT3-associated signaling pathways using cell-penetrating, cationic peptide constructs.
  • Acute, reversible pathway modulation in cultured cells and exploratory phenotypic screening within compound libraries.
• Practical use considerations (general):
  • Prepare sterile-filtered stock solutions and aliquot to avoid freeze-thaw.
  • Titrate concentration across a broad range (e.g., 0.1–20 μM final) to establish non-toxic working windows; monitor cell viability.
  • Include peptide control(s), such as scrambled or CPP-only sequences, to distinguish sequence-specific effects (if available).
  • Time-course experiments (e.g., 0.5–24 h) can resolve early vs late signaling effects.
• Readouts (general examples):
  • Western blot or ELISA for phospho-STAT3, reporter assays, or qPCR for downstream gene expression changes.
• Important: Verify sequence, purity, and counterion content on the CoA/Spec Sheet before designing assays; activity is sequence- and context-dependent.

Reaction Conditions

Classical bench reactions are not applicable. The following summarizes general, literature-based experimental conditions for using CPP-fused peptide tools in cell-based assays. These are not item-specific specifications.

• Stock preparation (general):
  • 1–10 mM in sterile water, dilute acid, or DMSO, depending on solubility; aliquot and store at −20°C protected from light under inert gas (matches item storage guidance).
• Working concentrations (typical literature ranges):
  • 0.1–20 μM in cell culture; optimize per cell type and endpoint. Include vehicle controls and CPP-only/scrambled peptide controls when available.
• Incubation conditions:
  • 30 min to 24 h exposure at 37°C with 5% CO2 for mammalian cells; shorter pulses can be used for acute signaling readouts.
• Compatibility:
  • Serum-containing media can influence uptake and stability; compare serum-free vs serum-containing conditions empirically.
• Readouts:
  • Western blot for phospho-protein levels, reporter assays, immunofluorescence for nuclear translocation, qPCR for target gene expression.

These are general guidance points. Validate and optimize for your specific biological system.

Safety & Handling

• GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS.
• General precautions for peptide research reagents:
  • Avoid inhalation of powders and contact with skin/eyes; handle in a chemical fume hood or biosafety cabinet as appropriate.
  • Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves; use dust mask/respirator per risk assessment when weighing lyophilized powder.
  • Prevent aerosolization during reconstitution; wet the vial walls with solvent before vigorous mixing.
• Incompatibilities (general): strong oxidizers; avoid extreme pH or elevated temperatures that can promote peptide hydrolysis or side-chain modification.
• Peroxide formation: not a concern for peptide solids (applies to certain ethers; not relevant here).
• First aid overview (general): rinse eyes/skin with water for at least 15 minutes upon contact; seek medical attention if irritation persists. If inhaled, move to fresh air; if ingested, rinse mouth and seek advice. Always follow institutional EHS protocols.
• Waste: dispose according to local regulations for organic laboratory chemicals/biochemical reagents.
• Shipping: Shipped in ice chest with ice pads (per product data). Inspect upon receipt; allow to equilibrate in desiccator before opening to limit moisture uptake.
• Always consult the SDS for authoritative hazard, exposure limits, and emergency measures.

Solvent Selection

This product name indicates a peptide acetate salt; solvent selection below reflects general practices for cationic/CPP-containing peptides. Item-specific solubility is not provided; verify empirically at small scale.

• Primary choices (general/literature):
  • Water or buffer (e.g., PBS, HEPES): Often effective for cationic peptide acetates; start at 1–5 mg/mL.
  • Dilute acid: 0.1–1% acetic acid or 0.01–0.1 M HCl can aid initial wetting/dissolution, followed by dilution with buffer to target pH.
  • DMSO: Useful to prepare concentrated stocks (e.g., 10–50 mM) for later dilution; check biological tolerance to DMSO.
• Co-solvent strategy: If partial solubility is observed, add 5–20% water to DMSO or vice versa, then stepwise dilute into buffer while gently mixing to avoid precipitation.
• Avoid: high ionic strength or basic pH during initial dissolution, which can promote aggregation of cationic peptides.
• Filtration: Use low-protein-binding 0.22 μm filters (PVDF/PTFE) if sterile solutions are required; pre-wet filters with solvent to minimize adsorption.
• Small comparison (general):
  • Water/buffer: most biocompatible; may have limited solubility for hydrophobic sequences.
  • DMSO: maximal solubilizing power; requires careful dilution to prevent precipitation and to maintain cell viability.
  • Acidified water: improves protonation/solubility; adjust pH post-dissolution to experimental conditions.

Storage & Reconstitution

• Storage conditions (item-specific from product data):
  • Store at −20°C.
  • Protect from light.
  • Argon charged.
  • Shipped in ice chest with ice pads; refrigerate/freezer-store upon receipt.
• Shelf-life: Not specified for this item; refer to CoA/Spec Sheet.
• Reconstitution (general guidance for peptide acetate salts):
  • Allow vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.
  • Briefly centrifuge to collect material at the bottom of the vial.
  • Dissolve initially in sterile water, dilute acetic acid (0.1–1%), or DMSO depending on solubility; target 1–10 mM stock solutions.
  • If dissolved in acidified water, adjust to working pH with sterile base after complete dissolution.
  • Sterile-filter (0.22 μm, low-protein-binding) if required.
• Aliquoting:
  • Prepare single-use aliquots to avoid repeated freeze–thaw; store aliquots at −20°C or below, protected from light, under inert atmosphere if feasible.
• Handling notes:
  • Avoid vigorous vortexing that can cause foaming/air exposure; mix gently.
  • Record exact solvent and concentration on the vial; verify by weight or amino-acid analysis when critical.

For any item-specific solubility, concentration limits, or stability in solution, consult the CoA/Spec Sheet.

Structure & Identity

• Item identity is peptide-based by name; detailed structure is not provided in the product data and should be confirmed from the CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• CAS: A413941 (internal placeholder; not a registry number).
• Structural features (general/literature-based interpretation):
  • The name “APTSTAT3-9R acetate” suggests a peptide comprising an APTSTAT3 motif linked to a nona-arginine (9R) cell-penetrating sequence, isolated as the acetate salt. This interpretation is based on common peptide-naming conventions in the literature and is not an item-specific confirmation.
  • If applicable, such constructs typically contain multiple guanidinium groups (from arginine side chains) and amide bonds (peptide backbone).
• 2D description (general): linear polypeptidic chain with cationic residues; acetate counterions balance positive charge in salt form.
• Category: Small molecules and compound libraries (as listed), though the name implies a peptide tool compound.
• Verify exact amino-acid sequence, termini (acetylation/amidation), and counterion content from the CoA prior to use.

Synthetic Utility

This product is a peptide tool compound rather than a building block for routine synthetic transformations, so classical synthetic utility is limited.

• Potential uses in chemical biology workflows (general):
  • As a probe in pull-down/competition experiments to interrogate protein–protein interactions associated with STAT3-related pathways.
  • As a positive or negative control in cell-penetration studies using CPP motifs (e.g., poly-arginine sequences), to benchmark uptake.
• Derivatization (general):
  • If sequence information and termini are known (consult CoA), chemoselective labeling (e.g., N-terminal acylation, lysine side-chain modification, or click chemistry if an azido/alkyne handle is present) can generate fluorescent or biotinylated analogs for imaging or affinity capture.
  • Conjugation strategies should preserve CPP integrity and avoid quenching uptake (excessive hydrophobic fluorophores may alter behavior).
• Retrosynthetic note:
  • Solid-phase peptide synthesis (SPPS) with orthogonal protecting groups is the standard route for such constructs; this is noted for context only and not specific to this item.

For precise reactivity or modification options, the exact sequence and protecting group state must be confirmed from the CoA/Spec Sheet.

Target Specificity

No target specificity data are provided in the product listing for this item.

• Antigen/target name: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity and epitope/sequence details: Not specified for this item; refer to CoA/Spec Sheet.
• Clone/isotype (if antibody): Not applicable.

Note: The product name suggests relevance to STAT3-related research in literature, but no item-specific binding or selectivity information is supplied. Users should consult the CoA or perform their own target engagement assays (e.g., CETSA, pulldown, reporter modulation) as appropriate.