Calpain-1, CAS No.689772-75-6

CAS: 689772-75-6 Cat. No.: np001115

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Porcine Erythrocytes; ≥1000 U/mg protein; Protein concentration: See COA

Accession #

P35750

Bioactivity

≥1000 U/mg protein

Overview

 Native calpain-1 from porcine erythrocytes. Calpains are a family of calcium-dependent thiol-proteases that degrade a wide variety of cytoskeletal, membrane-associated, and regulatory proteins. The two major isoforms, calpain I (µ-form) and calpain II (m-form), differ in their calcium requirement for activation. Calpain I requires only micromolar amounts of calcium (EC50 = 2 µM), while calpain II requires millimolar amounts (EC50 = 1 mM).

Calpains are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme. The 30 kDa unit is a regulatory subunit and common to both calpain I and calpain II. The 80 kDa unit consists of four domains (I-IV). The 30 kDa unit has two domains (V and VI).

• Domain I is partially removed during autolysis.

• Domain II is the protease domain.

• Domain III exhibits a homology with typical calmodulin binding proteins and interacts with calcium binding domains (IV and VI) and frees domain II for protease activity.

• Domain IV is a calcium binding domain.

• Domain V contains a hydrophobic region and is essential for calpain interaction with membranes.

• Domain VI is a calcium binding domain.

More recently, attention has been focused on the pathological significance of calcium accumulation in the central nervous system following cerebral ischemia and traumatic brain injury. Over-activation of NMDA, kainate, and AMPA receptors in the brain leads to sustained influx in Ca²⁺ through voltage gated Ca²⁺ channels. Disturbances in calcium homeostasis result in the activation of several calcium-dependent enzymes including calpains. Over-expression of calpains has been positively linked to both acute and chronic neurodegenerative processes including ischemia, trauma, and Alzheimer′s disease. In Alzheimer′s disease the ratio of active (76 kDa) to inactive (80 kDa) calpain I is reported to be much higher. Calpain proteolysis is usually the late-stage common pathway towards cell death induced by excitotoxic compounds.

Specifications

Product Name

Calpain-1, CAS No.689772-75-6

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Porcine Erythrocytes; ≥1000 U/mg protein; Protein concentration: See COA

Biochemical and Physiological Mechanisms

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1. Cleaves and activates caspase-7 (CASP7). Catalytic activity: Broad

Bioactivity

≥1000 U/mg protein

Accession #

P35750

Predicted molecular weight

110000

CAS

689772-75-6

Enzyme Commission Number

EC 3.4.22.52

Molecule Type

Enzyme

Storage and Shipping

Shape

20 mM imidazole-HCl, 5mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.

Concentration

from Porcine Erythrocytes; ≥1000 U/mg protein; Protein concentration: See COA

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at -80℃ long term. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorogenic. Note: 1 caseinolytic unit = 9 fluorogenic units.