Crystal Violet Aqueous Solution (5%) - BioReagent,Suitable for microbiology,Biological Stain,for microscopy,5% , CAS No.548-62-9

CAS: 548-62-9 Cat. No.: C1520409 Formula: C₂₅H₃₀N₃Cl Molecular Weight: 407.98 EC Number: 208-953-6

GRADE & PURITY BioReagent, Suitable for microbiology, Biological Stain, for Microscopy, 5%

Synonyms

5% Gentian Violet Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining, Microbial staining

Overview

  Under normal circumstances, bacteria are easily stained with basic dyes. The chromogenic ions of basic dyes carry a positive charge after ionization and readily bind to negatively charged stained substances. Since the isoelectric point of bacteria ranges from pH 2 to 5, bacteria carry a negative charge in alkaline, neutral and weakly acidic environments, and are thus prone to combining with positively charged dyes for coloration. Commonly used dyes include basic fuchsin, crystal violet and methylene blue.

Crystal violet, also known as methyl violet, has a molecular formula of C₂₅H₃₀N₃Cl·9H₂O, a molecular weight of 407.98 and a CAS number of 548-62-9. Together with methyl green, neutral red, methylene blue, safranin, hematoxylin, malachite green, methyl violet and basic fuchsin, it is a commonly used synthetic basic dye at present. It is soluble in water, ethanol and chloroform, but insoluble in diethyl ether. Its aqueous and ethanol solutions appear deep purple, and turn orange when exposed to concentrated sulfuric acid. It is used as an acid-base indicator with a pH color change range of 0.1 (green) to 2.0 (blue), a non-aqueous titration indicator in analytical chemistry, a biological stain and a chromogenic agent in colorimetric determination. Crystal violet is also an important dye for Gram staining of bacteria.

Operating Procedures (For Reference Only):

1. Sample Preparation

① For paraffin sections:

Deparaffinize in xylene for 5–10 min.

Replace with fresh xylene and deparaffinize for another 5–10 min.

Treat with absolute ethanol for 5 min.

Treat with 90% ethanol for 2 min.

Treat with 70% ethanol for 2 min.

Rinse with distilled water for 2 min.

② For frozen sections:

Rinse with distilled water for 2 min.

③ For cultured cells:

Fix with 4% paraformaldehyde for at least 10 min.

Wash with distilled water for 2 min.

Replace with fresh distilled water and wash for another 2 min.

2. Crystal Violet Staining

① Stain with crystal violet staining solution for 5–10 min; adjust the time according to staining results and requirements.

② Wash thoroughly with distilled water or tap water, then observe and photograph.

Staining Result:

Tissues or cells – Deep purple

Precautions: 

1. Crystal violet is harmful to the human body; proper protective measures are recommended.

2. It is advised to conduct a pre-experiment with 1–2 samples when using this reagent for the first time.

3. For your safety and health, wear a lab coat and disposable gloves during operation.

4. This product is for research use only and shall not be used for any other purposes.

Specifications

Synonyms

5% Gentian Violet Solution

Specifications & Purity

BioReagent,Suitable for microbiology,Biological Stain,for microscopy,5%

Stability And Storage

Store at room temperature long term (12 months). Store in the dark.

Storage

Protected from light,Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy, Suitable for microbiology

Product Line

Biological Products

Names and Identifiers

Isomeric SMILES

CN(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-]

Molecular Weight

407.98

Reaxy-Rn

3580948

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3580948&ln=

Chemical and Physical Properties

• Sensitivity: Light-sensitive
• Melt Point(°C): 215°C

Frequently Asked Questions

What does Suitable for microbiology mean?

Suitable for microbiology indicates that the product has been processed and tested for extraction workflows and microbiology. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 548-62-9.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.