306Oi10 Selective OGA Inhibitor: Key Facts & FAQs

306Oi10 is a selective small-molecule inhibitor of O-GlcNAcase (OGA), the enzyme responsible for removing O-linked N-acetylglucosamine (O-GlcNAc) modifications from proteins, making it a valuable tool for studying O-GlcNAc signaling in cellular and disease research contexts.

Product Name

306Oi10

Catalog Number (Aladdin)

O647772

Reference Catalog Number

MCE HY-151507

Primary Target

O-GlcNAcase (OGA)

Mechanism of Action

Selective inhibition of OGA, elevating cellular O-GlcNAc levels

Research Application

O-GlcNAc signaling, epigenetics, metabolic disease, neurodegeneration research

What Is 306Oi10 and How Does It Work?

306Oi10 (Aladdin SKU: O647772; also referenced as MCE HY-151507) is a selective inhibitor of O-GlcNAcase (OGA), a glycoside hydrolase enzyme that cleaves O-linked N-acetylglucosamine (O-GlcNAc) from serine and threonine residues on target proteins. By inhibiting OGA, 306Oi10 prevents the removal of O-GlcNAc modifications, thereby elevating global intracellular O-GlcNAc levels. This mechanism allows researchers to probe the functional consequences of enhanced O-GlcNAcylation across diverse biological systems, including metabolic regulation, transcription, and cell stress responses.

O-GlcNAc cycling is controlled by two enzymes: OGA (which removes the modification) and O-GlcNAc transferase (OGT, which adds it). Selective OGA inhibitors such as 306Oi10 enable precise pharmacological control of this cycle without directly interfering with OGT activity.

Research Applications of 306Oi10

306Oi10 is used as a chemical biology tool in cell-based and biochemical experiments requiring elevated O-GlcNAc signaling. Key application areas include:

• Neurodegeneration research: O-GlcNAc modifications on tau protein are linked to Alzheimer's disease pathology; OGA inhibitors help investigate the interplay between O-GlcNAcylation and tau hyperphosphorylation.
• Metabolic disease: O-GlcNAc cycling is sensitive to glucose flux through the hexosamine biosynthesis pathway, making OGA inhibitors relevant to diabetes and insulin resistance studies.
• Epigenetics and transcription: Numerous transcription factors and histones are O-GlcNAcylated; 306Oi10 can be used to study how elevated O-GlcNAc affects gene expression programs.
• Cancer biology: Aberrant O-GlcNAcylation has been observed in multiple tumor types, and OGA inhibitors serve as research probes in this context.

Researchers should validate the appropriate working concentration and treatment duration empirically for each cell or tissue system.

Handling, Storage, and Preparation Guidelines

As with most small-molecule research reagents, proper handling of 306Oi10 is essential to maintain activity and reproducibility:

• Storage: Store the compound according to the supplier's datasheet recommendation, typically at -20 °C under desiccated, light-protected conditions. Specific storage conditions for 306Oi10 should be confirmed on the Aladdin product page for O647772.
• Solubility: Solubility data specific to 306Oi10 should be verified on the product datasheet. Many OGA inhibitors are soluble in DMSO; prepare stock solutions accordingly and aliquot to avoid freeze-thaw cycles.
• Vehicle controls: Always include appropriate solvent vehicle controls in biological assays to distinguish compound-specific effects from solvent effects.
• Handling precautions: Handle as a potentially bioactive compound; refer to the Safety Data Sheet (SDS) for personal protective equipment (PPE) requirements.

For lot-specific purity, molecular weight, and analytical data, download the Certificate of Analysis (CoA) from the Aladdin website using the lot number printed on the vial label.

Ordering 306Oi10 from Aladdin Scientific

306Oi10 is available from Aladdin Scientific under catalog number O647772. To place an order or request a quotation:

• Search for O647772 or 306Oi10 on the Aladdin Scientific website.
• Select the required pack size and add to your cart, or contact your regional Aladdin sales representative for bulk pricing.
• For institutional purchase orders, billing, or invoicing queries, refer to the Payment, Pricing and Promotions section of the Aladdin Help Center.
• Shipping conditions and lead times vary by region; consult the Shipping and Delivery section or contact customer service for your specific location.

Registered account holders can track orders, download CoAs, and access invoices through the Manage Your Account portal.

Frequently asked questions

What is the primary biological target of 306Oi10?

306Oi10 selectively targets O-GlcNAcase (OGA), the enzyme that removes O-GlcNAc modifications from proteins. By inhibiting OGA, it raises intracellular O-GlcNAc levels, enabling researchers to study the downstream effects of enhanced O-GlcNAcylation.

What is the Aladdin catalog number for 306Oi10?

306Oi10 is listed under Aladdin Scientific catalog number O647772. It is also cross-referenced as MCE HY-151507. Use either identifier when searching on the Aladdin website or when contacting customer service.

In which solvent should 306Oi10 be dissolved?

Solubility information specific to 306Oi10 should be verified on the product datasheet or Certificate of Analysis available on the Aladdin website. Many small-molecule OGA inhibitors are soluble in DMSO; however, confirm solvent compatibility and maximum DMSO concentration tolerated by your biological system before use.

How should 306Oi10 be stored to maintain stability?

General best practice for small-molecule inhibitors is storage at -20 °C in tightly sealed, desiccated, and light-protected vials. Aliquot stock solutions to minimize freeze-thaw cycles. Always confirm the specific storage conditions stated on the Aladdin product page or CoA for O647772.

Can 306Oi10 be used in in vivo studies?

Whether 306Oi10 is suitable for in vivo use depends on its pharmacokinetic and toxicology profile, which should be detailed in the supplier's datasheet. Researchers planning in vivo experiments should review all available preclinical data and conduct appropriate pilot studies. Contact Aladdin Scientific customer service for the most current technical documentation.

References

1. MCE (MedChemExpress) product entry for HY-151507 (306Oi10). MedChemExpress, accessed 2024.
2. Vocadlo DJ. O-GlcNAc processing enzymes: catalytic mechanisms, substrate specificity, and enzyme regulation. Current Opinion in Chemical Biology. 2012;16(5-6):488-497. doi:10.1016/j.cbpa.2012.10.021
3. Hart GW, Slawson C, Ramirez-Correa G, Lagerlof O. Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease. Annual Review of Biochemistry. 2011;80:825-858. doi:10.1146/annurev-biochem-060208-092911

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.