Animal model of acute disseminated intravascular coagulation

Summary

The animal model of acute disseminated intravascular coagulation is an animal disease model induced by endotoxin. Currently, there are 1 main methods used to construct an animal model of acute disseminated intravascular coagulation: endotoxin-constructed acute disseminated intravascular coagulation animal model.

Principle

The basic principle of the animal model of acute disseminated intravascular coagulation is that the disturbance of the balance between coagulation function and anticoagulation function of the blood system is the main pathogenesis of disseminated intravascular coagulation (DIC). Clinically, severe infection is a common cause of DIC, and the use of endotoxin causes a systemic Schwartzman reaction in animals, i.e., by injecting the culture fluid of enteric typhus bacteria into the body of rabbits twice, DIC is induced. The use of endotoxin causes a systemic Schwartzman reaction in the animal, i.e., by injecting the culture of S. enterica twice into the rabbit, the balance between coagulation and anti-susceptibility of the rabbit's blood system is disrupted, and DIC is induced.

Appliance

The common application areas of animal models of acute disseminated intravascular coagulation are as follows: for exploring the pathogenesis of DIC and its preventive and control measures and the effect evaluation study of drugs, also can be used to carry out the differential study of the toxicity of strains and agents, the study of endotoxin lethality of various genera of animals, target organs, and the differential study of the animal's sensitivities to endotoxin.

Operation method

Construction of an animal model of acute disseminated intravascular coagulation by endotoxin

Principle

The basic principle of constructing an animal model of acute disseminated intravascular coagulation with endotoxin is to use endotoxin to cause a systemic Schwartzman reaction in animals, i.e., by injecting the culture fluid of S. Enteritidis twice into rabbits, the coagulation function of the rabbit's blood system is disrupted in equilibrium with the antisusceptibility function, and the occurrence of DIC is induced.

Materials and Instruments

Equipment:
Syringes, blood collection needles, etc.
Reagents:
①Escherichia coli endotoxin
② Salmonella endotoxin
③ Phenobarbital sodium.

Move

The basic process of constructing an animal model of acute disseminated intravascular coagulation by endotoxin can be divided into the following steps:

A. Rabbit, female or female.

B. Systemic Schwartzman reaction: Escherichia coli endotoxin (or Salmonella endotoxin at a dose of 25-200 mg/kg) is slowly injected into rabbits at a dose of 25-200 mg/kg into a marginal ear vein at intervals of 21-24 hours for 2 times;

C. Continuous injection of endotoxin: after anesthetizing rabbits with sodium phenobarbital, insert a catheter into the marginal ear vein of rabbits, and inject endotoxin at a rate of 30-50 µg/(kg-h) for 10-14 consecutive hours, and observe the clinical manifestations of rabbits with DIC, peripheral platelet counts, coagulation time and so on.

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