The main pathological feature of bronchopulmonary dysplasia is the obstruction of alveolar and pulmonary vascular development, which is manifested by the decrease in the number of alveoli, the increase in their size, and the simplicity of alveolar structure. More and more animal experiments and clinical studies have shown that hyperoxia is an important factor leading to the occurrence and development of bronchopulmonary dysplasia (BPD) in newborn infants, and therefore animal models with hyperoxia exposure are often used to study the mechanism of BPD.
Operation method
Establishment of a new animal model of bronchopulmonary dysplasia
Materials and Instruments
Animal model: Move The modeling method of the new bronchial time sterile animal model is as follows: . 10-12 newborn rats of each group in each litter, together with their surrogate mothers, were placed in a homemade oxygen box with a temperature of 25-26 ℃ and a humidity of 60%-65%. B. Oxygen concentration was maintained at 60% by an oxygen meter, and calcium lime was placed inside the box to absorb CO2 and prevent the CO2 concentration in the box from being too high. C. The air group was placed in the same room air, and the feeding conditions were the same as the high oxygen group. D. Open the box for 1 hour every day, weigh the newborn rats, exchange the female rats in the oxygen box with the air control group to prevent oxygen poisoning of the female rats, and replace the feed, water and bedding, and closely observe the survival of the rats in each group in terms of activity, respiration rate, lip color, and so on. E. Checked after 4 and 14 days of oxygenation, oxygen dependence appeared, and the lung tissue showed disturbed alveolar structure. Alveolar enlargement and thickening of intervals (4 days) or enlargement of alveolar lumen, simplification of structure, thickening of intervals, and reduction in the number of alveoli (14 days) were judged to be qualified BPD models. . Ten rats were randomly selected from each group after 4 and 14 days of oxygenation, respectively, and anesthetized by intraperitoneal injection of 10% hydralazine (0.3 mL/g). B. The thoracic cavity was dissected open, and the lungs were intubated to keep the lungs inflated, and the color and size of the lung tissues were observed to see whether there was hemorrhage, necrosis and edema. , The thoracic cavity of rats was dissected open, and after the whole lung was perfused with saline, pre-cooled 4% paraformaldehyde was injected from the left bronchus into the lungs until they were inflated to the tip of the lungs. B. The left lung was fixed in paraformaldehyde fixative for 24 hours, then dehydrated with gradient ethanol, embedded in paraffin, and paraffin sections (4 μm thick) were made, and 3 consecutive paraffin sections were taken from each specimen. C. Hematoxylin-eosin (HE) staining was performed, and the histopathological changes in the lungs of rats in each group were observed under light microscope. For more product details, please visit Aladdin Scientific website.
Neonatal mouse.
Reagents:
①4% paraformaldehyde;
② Alcohol;
③ Saline;
③ Saline. ④ 10% hydrated oxaldehyde;
⑤ Ethanol;
⑥ Paraffin.