Animal models of vitamin D deficiency

Summary

The main function of vitamin D (Vitamim D.VD) is to regulate calcium and phosphorus metabolism in the body and maintain normal growth and development of teeth and bones. Vitamin D deficiency can not only lead to vitamin D deficiency rickets, but is also associated with immune disorders, cancer, cardiovascular disease, asthma, and mental illness. By reducing vitamin D intake and skin synthesis of vitamin D, it is possible to establish an animal model of vitamin D deficiency.

Principle

Vitamin D deficiency modeling by reducing vitamin D intake

Appliance

It is mainly used in the study of vitamin D deficiency and its related various diseases.

Operation method

Establishment of an animal model of vitamin D deficiency

Principle

Vitamin D deficiency modeling by reducing vitamin D intake

Materials and Instruments

Reagents:
① Corn meal 76%;
② Wheat bran 20%;
③ Calcium carbonate 3%;
④ Iodized salt 1%;
⑤ Lysine 0.59%;
(vi) B vitamins.

Move

The modeling of the vitamin D deficiency animal model is based on the type of rats as follows:

(I) Wistar rat model A

. Weaned Wistar rats of 21 days of age, half male and half female, were fed with vitamin D deficient diet. The rats were fed with vitamin D-deficient diets. They were protected from light and fed in a single stainless steel plastic cage for 30 days.

B. The success of modeling was confirmed by measuring the blood biochemical changes and X-ray performance of the rats.

C, Serum alkaline phosphatase (AKP) was increased, blood calcium and blood phosphorus were decreased, bone X-ray showed blurred early calcification bands, softened bone, obviously small bones, decreased bone density, thinned and blurred bone trabeculae, and thinned bone cortex. Under the light microscope of bone tissue, the layer of hypertrophic chondrocytes was increased, the cell column was twisted, and the cartilage island protruded to the stem side in a focal distribution, which made the line of ossification irregular.

(B) BALB/c mouse modelA. BALB/c mice at 2 weeks of pregnancy were fed vitamin D-deficient diets and free water, and raised in a single cage.

B. The cages were placed in an ultra-clean workbench, which was covered internally with a door panel and externally with an opaque curtain, ventilated once every 12 hours, and irradiated for 12 hours during the day with a yellow light that did not contain ultraviolet radiation, and then extinguished at night.

C. Pregnant rats give birth to offspring in 6 days or so, and the offspring are weaned at 3 weeks after birth and kept in the same environment as the mother rats. The weights of the mice were measured at 4-10 weeks and 12 weeks. At 6 weeks, the mice began to show a decrease in their activity level and to walk on the ground.

D. Mice were killed at 12 weeks. Blood was collected from the tails of the mice, and a significant decrease in serum vitamin D level was measured, indicating successful modeling.

For more product details, please visit Aladdin Scientific website.

https://www.aladdinsci.com/