Dissolve 58.43grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Component
g/liter
Yeast Extract .
7.5
Dextrose
10
Casein Digest Peptone.
10
Sodium Citrate
5
Potassium Phosphate
5
Sodium Chloride
5
Tween 80
0.2
Sodium Carbonate
1.25
Magnesium Sulfate
0.8
Magnesium Chloride
0.14
Ferrous Sulfate
0.04
Agar
13.5
Total: 58.43g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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