Protocols

Bradford's Reagent Recipe

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve 50mg of Coomassie Blue G250 in 50ml of methanol

2.Add 100ml of 85% H3PO4 to the solution from step 1

3.Add the solution from step 2 into 500ml of H2O and mix

4.Filter to remove and precipitates

5.Add an additional 350ml of H2O

6.Store at 4℃.

Caution: Always add acid slowly into water and do not add water into acid.

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Bradford's Reagent Recipe" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/bradfords-reagent-recipe-en.html

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