Detection of apoptosis and other forms of cell death

Summary

Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".

Operation method

Detection of apoptosis and other forms of cell death

Move

Basic Scheme 1 Quantification of cell activity by live cells or fluorescent dyes

Microscopic observation is a simple and accurate method of detecting apoptosis. The hallmark features of apoptosis include a concentrated nucleus
shrinkage, intact but foaming cell membranes, and clear cytoplasm.

Materials (see Appendix 1 for 7 items)

V Dyestuff mixing agent

Cellular suspension of PBS (Appendix 1)

9 6-well round bottom plate

Microscope slides

No. 1 thickness coverslips or coverslips pre-coated with poly(L-lysine) (for non-adherent cells, Sigma)

Fluorescence microscope with 40x or 63x objective and appropriate filters (Table 2.14.1)

BASIC PROGRAM 2 Quantification of DNA Cleavage Fragments

This method was used to study the quantification of DNA cleavage fragments in cytotoxic T cells (C TL, unit 2.10) and natural killer cells (N K cells, unit 2.10), and to study the quantification of DNA cleavage fragments in cytotoxic T cells.
(Unit 8.6) mediated apoptosis.

Materials


Options for counting DNA fragments in radioactively labeled DNA quantification cells Additional material


Supplementary Option 1 Radiolabeling of cell nuclei with 125I UdR and 51C r Materials

Cell suspension
V R P M I -10 complete medium
Water-soluble lmCi/ml iodine (125I U d R ) (approx. 2000 Ci/m m o l ; ICNBiomedicals)

Supplementary Option 2 Radiolabeling of cell nuclei with 3H TdR Materials

For more product details, please visit Aladdin Scientific website.

https://www.aladdinsci.com/