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Does the protein's own charge affect the buffer?
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Does the protein's own charge affect the buffer?
No. The SDS in the buffer binds to the protein and completely covers the protein-SDS complex. The SDS in the buffer binds to the protein and causes the protein-SDS complex to take on a large negative charge, completely covering the protein's own charge.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "Does the protein's own charge affect the buffer?" Aladdin Knowledge Base, updated Jun 9, 2025. https://www.aladdinsci.com/us_en/faqs/does-the-protein-own-charge-affect-the-buffer-en.html
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