Giemsa Stain

Recipe

Stock Solution

1.Dissolve 3.8g of Giemsa powder into 250ml of methanol

2.Heat the solution from step 1 to ~60oC

3.Slowly add in 250ml of glycerin to the solution from step 2

4.Filter the solution from step 3

5.The solution needs to stand a period of time prior to use. Although times vary based on who you ask a minimum of two months is usually recomended

Working Solution

1.Add 10ml of stock solution to 80ml of distilled water and 10ml of methanol