HPGDS Antibody: FAQs, Applications & Specifications

HPGDS Antibody is a research-grade immunological reagent targeting hematopoietic prostaglandin D synthase, a key enzyme in the prostaglandin D2 biosynthesis pathway with relevance to inflammation, allergy, and immune-related research.

SKU

Ab351243

Product Name

HPGDS Antibody

Formulation

PBS only (no carrier protein, no azide indicated)

Clone/Source Reference

absea #OC-220

Target Protein

HPGDS (Hematopoietic Prostaglandin D Synthase)

Supplier

Aladdin Scientific

What Is HPGDS and Why Is It Studied?

HPGDS (Hematopoietic Prostaglandin D Synthase) is a cytosolic enzyme belonging to the sigma-class glutathione S-transferase family. It catalyzes the isomerization of prostaglandin H2 (PGH2) to prostaglandin D2 (PGD2) in the presence of glutathione. PGD2 is a potent lipid mediator involved in inflammatory responses, allergic reactions, sleep regulation, and immune cell recruitment. HPGDS is predominantly expressed in hematopoietic cells, including mast cells, megakaryocytes, and Th2 lymphocytes, making it a key target in allergy, asthma, and inflammatory disease research.

Product Overview: HPGDS Antibody (Ab351243)

The HPGDS Antibody (SKU: Ab351243) available from Aladdin Scientific is referenced under absea catalog number OC-220. The antibody is supplied in PBS (phosphate-buffered saline) formulation. Researchers should consult the accompanying datasheet for detailed information on host species, clonality, isotype, and validated applications such as Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), or flow cytometry (FC). Always verify species reactivity and recommended dilution ranges before use.

Storage, Handling, and Stability

Antibodies formulated in PBS without stabilizing additives such as BSA or glycerol may require special attention during storage and handling. General best practices include: storing the antibody at 4 °C for short-term use (up to 1 month) and at -20 °C or -80 °C for long-term storage. Avoid repeated freeze-thaw cycles by aliquoting upon receipt. Do not add azide unless specified, as it can interfere with certain enzymatic detection methods. Refer to the official product datasheet for the exact recommended storage conditions for Ab351243.

Recommended Applications and Protocol Tips

HPGDS antibodies are commonly employed in techniques including Western blot for assessing protein expression levels, immunohistochemistry for tissue localization, and immunofluorescence for subcellular localization studies. For optimal results, researchers should: (1) perform antibody titration to determine the ideal working concentration; (2) include appropriate positive controls such as lysates from mast cell lines or tissues known to express HPGDS; (3) use validated secondary antibodies matched to the host species of the primary antibody; and (4) follow standard blocking protocols to minimize non-specific background. Specific validated applications and dilution recommendations for Ab351243 are listed on the product datasheet.

Ordering, Availability, and Customer Support

The HPGDS Antibody (Ab351243) can be ordered directly through the Aladdin Scientific website or by contacting our sales and technical support team. For bulk orders, custom conjugation requests, or questions regarding lead times and shipping conditions, please reach out to customer service. Aladdin Scientific is committed to providing high-quality research reagents with full technical documentation. If you require a Certificate of Analysis (CoA), Safety Data Sheet (SDS), or additional validation data for Ab351243, these documents can be requested from our support team or downloaded from the product page.

Frequently asked questions

What is HPGDS Antibody (Ab351243) used for?

HPGDS Antibody (Ab351243) is used to detect and study the expression and localization of hematopoietic prostaglandin D synthase in biological samples. Common applications include Western blot, immunohistochemistry, and immunofluorescence in research contexts related to inflammation, allergy, and immune cell biology. Please refer to the product datasheet for the complete list of validated applications.

How should I store the HPGDS Antibody to maintain its activity?

Since Ab351243 is formulated in PBS without additional stabilizers specified in the available source information, it is generally recommended to aliquot the antibody upon receipt and store aliquots at -20 °C or -80 °C for long-term preservation, while keeping working aliquots at 4 °C for up to one month. Avoid repeated freeze-thaw cycles. Always confirm exact storage conditions on the official product datasheet.

What species reactivity does the HPGDS Antibody cover?

Species reactivity information for Ab351243 is detailed on the Aladdin Scientific product datasheet and should be confirmed before experimental use. Typical HPGDS antibodies may be reactive against human, mouse, or rat samples, but specific reactivity must be verified from the official documentation to ensure compatibility with your experimental model.

What is the catalog reference absea #OC-220 listed on the product?

The reference absea #OC-220 indicates the originating manufacturer's catalog identifier from absea, which is the upstream supplier or OEM source for this antibody. Aladdin Scientific distributes this antibody under SKU Ab351243. Researchers can use the OC-220 reference to cross-check technical data or validation information available from the original manufacturer.

Can I use the HPGDS Antibody for flow cytometry?

The suitability of Ab351243 for flow cytometry (FC) depends on validated applications listed in the product datasheet, which should be consulted before experimental design. Not all antibodies formulated for standard immunoassays are validated for flow cytometry. Contact Aladdin Scientific technical support if you require guidance on protocol adaptation or confirmation of FC compatibility.

How do I request a Certificate of Analysis or Safety Data Sheet for Ab351243?

You can download the Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for HPGDS Antibody (Ab351243) directly from the Aladdin Scientific product page using the lot number printed on your product label. Alternatively, contact our customer service team with your order details and lot number to receive the relevant documentation by email.

References

1. Urade Y, Eguchi N. Lipocalin-type and hematopoietic prostaglandin D synthases as a novel example of functional convergence. Prostaglandins & Other Lipid Mediators. 2002;68-69:375-382.
2. Aladdin Scientific. HPGDS Antibody, Ab351243 Product Datasheet. Aladdin Scientific Inc.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.