Isolation and identification process experiments of enteric pathogenic bacteria from fecal specimens

Summary

Guiyang College of Traditional Chinese Medicine Basic Medicine Experimental Teaching Center Website

Operation method

Isolation and identification process experiments of enteric pathogenic bacteria from fecal specimens

Materials and Instruments

Patient fecal specimen S-S agar plate Disaccharide iron medium Typhoid and Shigella multivalent diagnostic sera Carrier plate

Move

1. Specimen collection: Use a sterile swab to take fresh, urine unmixed patient feces (pus and blood or mucus portion is better) and send it for immediate examination.

2. Inoculate the specimen directly on S-S agar plate, make zonal delineation for separation, and incubate in 37℃ thermostat for 18~24 hours.

3. pick colorless transparent or translucent colonies inoculated with iron bisaccharides medium, at 37 ℃ incubator culture 18 ~ 24 hours.

4. further according to the situation for the indole test, fat da test and other biochemical reactions.

5. Use multivalent diagnostic serum for serological identification.

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