The grouping, labeling and reasonable dyeing of experimental animals are the prerequisites for obtaining good experimental results and conclusions, and are also the first thing to be done in every toxicology experiment. Through this experiment, we will learn the scientific grouping, labeling and basic dyeing methods of test animals.
Operation method
Laboratory animal grouping, labeling, and dyeing experiments
Materials and Instruments
Adult healthy mice Move (i) Selection of healthy animals For more product details, please visit Aladdin Scientific website.
Crystalline violet Picric acid Magenta
Brushes, cotton swabs, animal scales, scales
Regardless of which species of genus and strain of animals are selected for the experiment, it is required to select healthy experimental animals. Healthy animals are required to meet the following requirements: full-bodied appearance, dense and glossy coat, close to the body surface, bright eyes, quick action, flexible response, good appetite and nutritional status.
(II) Identification of sex of experimental animals
Different sexes of animals have different sensitivities to toxicants, which may be related to sex hormones, hepatic microsomal hydroxylation reaction, and also varies with the test substance. Therefore, according to the experimental requirements to choose the gender, general experiments such as no special requirements for gender, to choose half of the male and female animals.
1. Rats and mice are mainly distinguished by the distance between the anus and the reproductive space, with the larger distance being male and the smaller being female.
2. guinea pig with one hand to grasp the guinea pig neck, the other hand to peel off the skin by the genital hole, male animals in the round hole to reveal the protrusion of the sex organs, female animals are now triangular gap, the adult female chest has two nipples.
3. domestic rabbit
(iii) the method of grasping experimental animals
1. The mouse is first lifted by the tail with the right hand, placed in the mouse cage or on the experimental bench and pulled backward, and when it is crawling forward, the left thumb and forefinger are used to grab the mouse by the two ears and the skin of the neck, and the mouse body is placed in the heart of the left hand to straighten the hind limbs, and the ring finger is pressed on the tail of the mouse, and the little finger is pressed on the hind legs.
2. The method of catching rats is basically the same as that of mice, but because rats are ferocious, it is not suitable to catch them by attacking methods. To avoid bites, put on canvas or cotton gloves. Using the left hand fixation method, the rat's ear was pinched with the thumb and forefinger, and the remaining three fingers pinched the rat's dorsal skin and placed it in the left palm, so that the right hand could be used for all kinds of experimental operations.
3. The guinea pig's grasping method is timid and easy to scare, when grasping, it should be stable, accurate and rapid. Use the palm of the hand to quickly buckle the back of the rat, grab it above the shoulder blade, hold the neck with the thumb and forefinger, and hold the buttocks with the other hand.
4. Grab the rabbit with the right hand to grasp the rabbit neck fur lifting, and then the left hand to support its hips or abdomen, so that most of the weight of its weight is concentrated in the left hand.
(iv) Random grouping method of experimental animals
1. Random number grouping method
2、Randomized grouping method
(E) the number of laboratory animals, labeling methods
1. The inductance of weighing weight and mouse scale should be below 0.1g. According to the different requirements of the experiment, a certain number of mice should be selected, and the weight requirement should be less than 10% of the average weight of animals of the same sex in the same group, and the difference in the average weight of animals of the same sex between different groups should be less than 5%.
2. Numbering
(1) Staining method: generally use picric acid. Generally, the head is 1, the right front leg is 2, the right waist is 3, the tail base is 5, the left back leg is 6, the left waist is 7, the left front leg is 8, and the back is 9.
(2) Ear-cutting method
(3) Branding method
(4) Number plate method
(F) Pathways and Methods of Poisoning of Laboratory Animals
1. Subcutaneous injection
2. Intradermal injection
3.Intramuscular injection
4. Intraperitoneal injection
5. Intravenous injection
(1) Rabbit: Commonly used vein at the outer edge of the ear;
(2) Mice: usually intravenous (left and right bilateral); 6. Oral poisoning: gavage, capsule, free-feeding.
7. Other routes of administration:
(1) Respiratory tract poisoning
(2) Skin poisoning: a. patch method; b. tail dipping method