Microscope use training experiment
Microscope use training experiment
The microscope is an important tool for mastering morphology, and it is necessary to master the performance and usage of each part. Due to the parasite examination specimens are mostly feces and other excreta, so the microscope table should not be tilted, so as not to lose the specimen, affecting the results of the examination, and contamination of the environment around the work.
Operation method
microscopic observation Move I. Remove the microscope with both hands and hold the microscope with the right hand on the arm and the left hand on the lens holder, keeping it in a horizontal position and placing it gently on the lab bench. Check and clean the mechanical and optical parts, and report to the instructor when there is a problem with the inspection. For more product details, please visit Aladdin Scientific website.
II. Use of low magnification microscope
1. For light turn the low magnification objective lens to the bottom of the barrel. Rotate the reflector so that external light is reflected into the reflector. When the light is strong, use a flat mirror, and when it is weak, use a concave mirror. If the luminosity is not enough, raise the spotter and open the aperture.
2. Adjust the focus by looking at the objective lens from the side, turn the coarse adjuster (large spiral) so that the lens barrel slowly down until the low-magnification mirror is about 2mm from the slide to stop, do not make the objective lens impact on the slide. Then put your eyes close to the eyepiece and observe the field of view while slowly raising the objective lens with the coarse adjuster. When the specimen appears in the field of view, adjust the focus with the fine adjuster (small spiral) until the specimen is clear.
III. Use of high magnification lens
High-magnification lens should be used on the basis of the use of low-magnification lens can be used, can not directly use high-magnification lens. Generally, the first use of low magnification to find the observation target, and the target with a specimen mover (push ruler) to the center of the field of view, conversion of high power objective, dimming, focusing to the object image clear after observation.
IV. The use of oil mirror
1. Place the mirror stage into a horizontal position.
2. Adjust the object with low magnification first and move it to the center of the field of view, adjust the aperture and spotter to increase the brightness of the field of view.
3. first drop a drop of mirror oil on the slide, and then convert the oil lens, looking from the side, with a coarse adjuster carefully move the oil lens down, so that the lens and the slide close to and submerged in the mirror oil, should pay attention to do not force too much damage to the slide and the oil mirror.
4. Eye shift to see the eyepiece, with a coarse regulator upward slightly elevated barrel, to see the outline of the specimen, switch to fine regulator adjusted to clear.
5. oil mirror with the end of the first will raise the barrel, remove the slide, with xylene-soaked mirror paper gently wipe off the mirror oil, until wiped clean. The specimen observed with the oil mirror should also be cleaned in time according to the above method. The microscope should be returned to its original place after use.
V. Microscope maintenance
Microscope is a valuable experimental equipment should be carefully cared for, before and after the experiment should be maintained and cleaned. The parts of the microscope should not be dismantled at will. If there is any abnormality, you should report to the teacher in time.
VI. The correct posture of microscope observation
Sit upright with your chest straight. Both eyes should be open and the specimen under the microscope should be observed with the left eye (or both eyes if binoculars are available). Both hands should be involved in the work, usually the left hand to adjust the focus, the right hand to move the slide or drawing. Observation should be focused, meticulous, and in a certain order.