Myocardial morphology observation experiment
Myocardial morphology observation experiment
Summary
This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Operation method
Myocardial morphology observation experiment
Move
1. Coloring: Hematoxylin
2. Visualization by the naked eye:
The specimen is part of the heart wall.
3. Low-magnification observation:
As the cardiac muscle fibers are not arranged in the same direction, there are longitudinal, transverse and oblique sections, so it is necessary to observe the specimen comprehensively and be familiar with the parts of various sections. Myocardial fibers are irregular and short cylindrical, with branches and anastomosing into a network for the longitudinal section, and round or circular small pieces for the transverse section.
4. High magnification observation:
Choose the longitudinal section of myocardial fibers with typical morphology to observe, and pay attention to distinguish it from skeletal muscle.
Longitudinal section: myocardial fibers are thinner and shorter than skeletal muscle fibers, and the branches anastomose into a network. Nuclei, located in the center of myofibers. Larger, sometimes double nuclei are seen. There are dark bands and bright bands composed of transverse striations. But not as obvious as skeletal muscle. Transverse muscle fibers in the cardiac muscle fibers at the beginning and end of the connection, can be seen with the transverse stripe parallel to the crimson straight line or step-like line for the intercalary disk.
Transverse section: myocardial fibers are round or irregular, similar in size. Myofibrils are dotted, reddish, and distributed in the periphery of myocytes. The nuclei are located in the center of the myofibers and are rounded, with some nuclei not seen. Myoplasm is very lightly colored, because the myoplasm is more at the ends of the nucleus, so in the end cut to the center of the nucleus discretionary cells are often seen in the light stained area.
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