The pentose phosphate pathway is an oxidative catabolism of glucose that produces NADPH and synthesizes ribose phosphate, which provides the raw material for nucleic acid metabolism. Glucose-6-phosphate dehydrogenase (G6PDH) is the key enzyme in the pentose phosphate pathway, catalyzing the oxidation of glucose-6-phosphate to glucose-6-phospholactone and the reduction of NADP* to NADPH. NADP* to NADPH for biosynthesis and maintenance of intracellular reduction. Therefore, the activity of G6PDH reflects the biosynthesis and antioxidant capacity of an organism to a certain extent.
Principle
The rate of metabolism of this pathway can be determined on the basis of NADPH production, and the rate of increase of NADPH at 340 nm reflects the enzymatic activity of G6PDH.
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