Preparation of EBV-transformed B cells

Summary

Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".

Operation method

Preparation of EBV-transformed B cells

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Basic Programs Materials

P M I - I O complete medium

B 95-8 cells (chimpanzee cell line; A T C C # C R L 1612), logarithmic growth phase
Peripheral heparin anticoagulated blood (Appendix 3G), tonsils, spleen cells or bone marrow

P B S

Ficoll-Hypaque solution

H B S S

lmg/m l Cyclin A, 100% (V/V) ethanol, stored at 20°C.

Beckman 4°C centrifuge and JS4.2 rotor (or alternative)

0.45um filter

50m l conical bottom centrifuge tube

25c m 2 cell culture flasks

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