Natural RNA library screening and protein-specific binding RNA sequence experiments
In cells, protein-RNA binding is involved in a variety of physiological regulation and developmental processes. Direct protein-RNA interactions occur during mRN
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Preparation of nuclear and cytoplasmic S100 extracts of HeLa cells for in vitro splicing reaction assay
Cytosolic extracts can be used for splicing of precursor mRNAs. The S100 extract, although it includes many splicing factors, does not have splicing activity be
Small-scale rapid preparation of Drosophila RNA
Guanidine hydrochloride rapidly inhibits RNAase activity while lysing cells, and this method utilizes this feature to isolate Drosophila RNA.
Preparation of yeast tRNA by phenol method
By purification on DEAE cellulose columns, small amounts of DNA, large molecular RNA, proteins, polysaccharides and other impurities were removed, and finally a
Preparation of yeast total RNA
Good quality total RNA can be extracted from yeast cells.
Purification of splicing factor SR protein assay
Precursor mRNA ( pre-mRNA ) splicing factors The SR (serine/argininc rich) family of proteins is an important player in the assembly of splices onto precursor m
Preparation of fungal total RNA
Total RNA was extracted from a variety of different fungi.
Yeast precursor mRNA splicing extract assay
The splicing of eukaryotic pre-mRNA is a two-step process, in which an intron is cut from the precursor and two exons are joined together to form the mature mRN
Total RNA or poIy(A) +RNA from Drosophila embryos
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)