Indirect immunofluorescence of living cells
The surface of living cells retains a more complete antigen or receptor, first use specific mouse-derived monoclonal antibody to bind to the corresponding antig
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Purification of antibodies from immune sera
Purified antibodies are often used for direct detection of antigens. In this case the antibody is labeled with an easily identifiable marker for binding and det
Isolation and exchange of Streptomyces crassus
Neurospora crassa (2n = 14), also known as red bread mold, taxonomically belongs to the Ascomycetes, coccolithophores, and pulsatilla genera of fungi, and four
in vitro transcription technology
Usually transcription occurs in the organism, if we will contain RNA transcriptase, NTP and other conditions, in an in vitro cell-free system, using DNA as a te
Chip Preparation Techniques for Direct Dot Sampling
The direct dispensing method, first developed by Brown Laboratory of Stanford University, involves arranging trace oligonucleotide fragments, cDNA or proteins,
Experimental modeling of heterologous in vitro fertilization
Heterozygous in vitro fertilization experimental modeling can be used to: (1) simulate in vivo conditions of male and female gametes from different species of a
Hybridoma cloning
Hybridoma cloning is mainly used to (1) clone antibody-positive holes. (2) Separating antibody-secreting cells, antibody-nonsecreting cells, desired antibody (s
Novel vector-mediated gene introduction technologies
Currently gene introduction technology is the main technique for the preparation of ips cells, and the improvement of gene vectors promotes the establishment of
Crystallization of lipid mesoporous membrane proteins
Crystallization of proteins using lipid mesoporous membrane proteins can be used to resolve X-ray crystal structures of prokaryotic and eukaryotic proteins, pro