Identification of IgG purity by polyacrylamide gel electrophoresis
Since the concentration of the polyacrylamide gel can be prepared on demand, two electrophoresis systems, the "Continuous System" and the "Discontinuous System,
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Dialyzed to obtain soluble refolding σ32 Monomer experiments
This experiment describes what dialysis conditions lead to the highest yield of soluble refolded σ32 monomer. This experiment is from the guide to protein
Experimental determination of protein concentration by bicinchoninic acid (BCA) assay
The bicinchoninic acid (BCA) assay is a new and improved Lowery assay that has recently been developed, with a simple reaction and virtually no influence of int
Heparanase Lowry colorimetric assay
The Lowry colorimetric method is one of the most sensitive methods for protein determination. The color development principle of this method is the same as that
Separation of lactate dehydrogenase isozymes by agarose gel electrophoresis assay
Lactate dehydrogenase is present in all glycolytic cells, and catalyzes the dehydrogenation of lactate to form pyruvate or the reduction of pyruvate to lactate
Experiment to determine the content of cell membrane proteins
Membrane proteins are protein molecules present in the cytoplasmic membrane of cells and play important roles in intracellular molecular transport, energy metab
Determination of key enzymes and important products in the anaerobic oxidation pathway
Glucose undergoes a 10-step metabolic reaction of glycolysis to produce keto acid, which is then further produced as lactate or decarboxylated into acetyl coenz
Determination of NADPH-cytochrome C (P-450) activity
NADPH-Cytochrome C (P-450) is a heme protein that is a terminal oxidase due to the fact that when it is in its reduced form, it combines with carbon monoxide to
Experiments for the determination of the isoelectric point of proteins
The isoelectric point of a protein is one of the important properties of proteins. Determination of isoelectric point of proteins is important for understanding