Laboratory Protocols & SOPs Library
- Experiments on purification of nucleic acids by ethanol precipitation with sodium acetate SummaryNucleic acids are water-soluble compounds of polyanions that can combine with many 1-valent and 2-valent ions to form salts, the latter of which do not dissolve or become invariant in organic solvents. The latter is insoluble and invariant in organic solvents. It forms a precipitate at ...
- Experiments on the effect of hemolysis and jaundice on the determination of enzyme activity SummaryTo examine the accuracy of enzyme activity measurement in the presence of interfering substances, this experiment is from the Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory tests.Operation methodExperiments on the effect of hemolysis and jaundice on the ...
- Protein-free hemofiltrate preparation experiment SummaryProtein-free hemofiltrate preparation experiments can be used for (1) precipitation of proteins by protein precipitants and (2) removal of precipitated proteins by filtration or centrifugation.Operation methodProtein-free hemofiltrate preparation experimentPrincipleExperimental principle ...
- Determination of glucose in serum by o-toluidine assay SummaryThrough this experiment, students can master the principle of o-toluidine method of blood glucose measurement, the preparation of reagents and learn the method of o-toluidine method of blood glucose determination. This experiment is from the laboratory guide of Mudanjiang Medical College ...
- Glycosylated serum protein assay SummarySerum glucose undergoes a non-enzymatic glycation reaction with the amino group at the N-terminus of albumin and other serum protein molecules to form a macromolecular ketamine structure. This ketamine structure can be reduced with nitrotetrazolium blue (NBT) in alkaline solution to form ...
- Serum Glucose Assay SummaryGlucose oxidase catalyzes the oxidation of glucose to glucuronic acid and produces hydrogen peroxide: glucose ten 2H2O + O2 = gluconic acid ten 2H2O2. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for laboratory tests.Operation methodSerum ...
- Experimental determination of total serum proteins by bis-urea method SummaryAll compounds containing two formamide groups (-CONH2-) in the molecule can interact with alkaline copper solution to form a purplish-red compound, this reaction is called the double urea reaction protein molecules have many peptide Jian (-CONH2-) can be this reaction, and the various ...
- Plasma fibrinogen assay SummaryThrough this experiment, trainees can master the principle and method of plasma fibrinogen determination and the preparation of reagents. This experiment is from the laboratory guide of Mudanjiang Medical College undergraduate 5-year testing program.Operation methodPlasma fibrinogen ...
- Serum Mucin Assay SummaryWhen 0.6 mol/L perchloric acid is used to determine the protein in serum, mucin is not precipitated and remains in the filtrate, then phosphotungstic acid is added to precipitate the mucin, and then the protein content is determined by phenol reagent.Operation methodSerum Mucin ...