Laboratory Protocols & SOPs Library
- VP Experimental SummaryThe VP assay can be applied to (1) identify Enterobacter aerogenes and Escherichia coli, and (2) characterize other bacterial groups, including actinomycetes.Operation methodBarritt's methodPrincipleCertain genera can decarboxylate pyruvic acid to produce neutral acetylmethylmethanol, ...
- Serum uric acid (UA) assay by phosphotungstic acid reduction assay experiment SummaryUric acid in deproteinized blood filtrate is oxidized to allantoin and carbon dioxide by tungstic acid in alkaline solution, and phosphotungstic acid is reduced to tungstic acid in this reaction, and the amount of tungstic acid generated is proportional to the content of uric acid in the ...
- Colorimetric assay for serum alkaline phosphatase (ALI) test SummaryTo enable students to master the principle of alkaline phosphoric acid determination, method and preparation of reagents, this experiment is from the Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory tests.Operation methodColorimetric assay for serum alkaline ...
- Urinary 17-hydroxysteroid assay SummaryUrinary 17-hydroxysteroids are hormones and their metabolites secreted by the suprarenal cortex, and their excretion from urine can reflect the function of the adrenal cortex. 56% sulfuric acid and ammonium sulfate were added to promote the transfer of 17-hydroxysteroids to the organic ...
- Serum amylase (AMS) assay test SummaryThrough this experiment, trainees can master the principle of amylase determination, the preparation of reagents and learn the determination method. This experiment is from the laboratory guide of Mudanjiang Medical College, undergraduate 5-year testing program.Operation methodSerum ...
- Experimental determination of serum creatinine (Cr) by the deproteinization endpoint method SummaryCreatinine in serum (plasma) reacts with alkaline picric acid to form a yellowish-red picric acid-creatinine complex, which is measured colorimetrically at 510 nm. This experiment is derived from the experimental guide of Mudanjiang Medical College, undergraduate 5-year laboratory testing ...
- Clear Aspartate Aminotransferase AST Assay SummaryAccording to this experiment, trainees can understand the reagent preparation of the experimental program and master its principle and measurement method. This experiment is from Mudanjiang Medical College, undergraduate 5-year laboratory guide for testing majors.Operation methodSerum ...
- Serum alanine transferase (ALT) measurement assay SummaryThis experiment enables students to master the principle of ALT determination. The reagent preparation method, this experiment is from Mudanjiang Medical College undergraduate 5-year laboratory guide for laboratory testing.Operation methodSerum alanine transferase (ALT) measurement ...
- Colorimetric assay for serum lactate dehydrogenase (LDH) assay SummaryThis experiment enables students to master the principle of colorimetric determination of lactate dehydrogenase, the preparation of reagents, and learn the method of LDH colorimetric determination. This experiment is from the laboratory guide of Mudanjiang Medical College undergraduate ...