Laboratory Protocols & SOPs Library
- Experiments on the isolation and identification of monocotyledonine SummaryEuphorbiaceae plant a leaf autumn, also known as the bottom of the leaf beads, also known as dog apricot strip, its roots, leaves and shoots contain a variety of alkaloids, the structure has been clear more than a dozen kinds. Among them, Ichibaqiu alkaloid has the highest content, and ...
- Experiments for the determination of blood concentration and half-life of drugs SummaryMeasurement of blood concentration and half-life of drugs is used (1) in order to guide the clinical selection of the optimal therapeutic regimen and the most appropriate therapeutic dosage for different individuals; (2) to improve the clinical drug efficacy; and (3) to reduce the ...
- Determination of pharmacokinetic parameters SummarySulfonamides containing p-aminobenzene in their structure can react diazotically with sodium nitrite in an acidic environment to produce a diazonium salt. This salt reacts with phenolic compounds (muscimol) in an alkaline environment to form an orange-colored azo compound. The shade of ...
- designed experiment SummaryThrough the creative design of a functional animal experiment (including pathological models of animals), within certain experimental conditions and scope, complete the whole process from experimental design to hands-on operation. In the course of the experiment, observe various ...
- Experimental effects of efferent nervous system drugs on blood pressure in anesthetized dogs SummaryUsing a direct blood pressure measurement method, an arterial cannula inserted into the common carotid artery and a pressure transducer form an anticoagulant closed system, and blood pressure values can be read from the BL-420 system connected to the pressure transducer to observe the ...
- Experimental action of cardiac glycosides on isolated frog hearts SummaryAfter the frog's heart is isolated, a frog heart cannula containing Ren's fluid is inserted into the ventricle, and this artificial perfusion method is used to keep the heart metabolism running smoothly in order to maintain the frog's heart contracting and diastole rhythmically. The heart ...
- Activity Screening and Activity Tracking Experiments of Traditional Chinese Medicines SummaryDPPH (2,2-diphenyl-1-picrylhydrazyl radical, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl) is a relatively stable lipid radical with a free electron on its N. Its ethanol solution is purple in color, with a maximum absorption peak at 517 nm. After the addition of antioxidants, DPPH ...
- Extraction, isolation and characterization of the alkaloids of Hantahexaenia japonica SummaryHan Fangji is the root of Stephania tetrandra S. Mcore (防己科千金藤属物), an expectorant, antipyretic and analgesic drug whose active ingredients are alkaloids. The main ones are hanfengzhi methylin and hanfengzhi ethylin. In addition to its clinical use in the treatment of ...
- Chinese medicine composition analysis and structure identification experiment SummaryThe objectives of this experiment are: 1. to master the basic principles and operation methods of high performance liquid chromatography (HPLC). 2. to master the application of HPLC analytical methods for the identification and determination of active ingredients of natural drugs. 3. to ...