Recombinant SORCS2 Antibody: FAQs & Technical Guide

Recombinant SORCS2 Antibody is a highly specific research reagent designed to detect and study the SorCS2 protein, a member of the Vps10p-domain receptor family implicated in neurotrophin sorting and neurological function.

Product SKU

Ab351395

Product Type

Recombinant Antibody

Formulation / Buffer

PBS (phosphate-buffered saline) only

Target Protein

SORCS2 (SorCS2, VPS10 domain-containing receptor 2)

Antibody Format

Recombinant (sequence-defined, lot-to-lot consistency)

Reference Clone / Catalog Series

absea#RN-085

What Is SORCS2 and Why Is It Studied?

SORCS2 (Sortilin-related VPS10 domain-containing receptor 2) is a type-I transmembrane protein belonging to the Vps10p-domain receptor family. It is predominantly expressed in the brain and plays roles in neurotrophin processing, synaptic plasticity, and intracellular protein sorting. Research implicates SORCS2 in neurological and psychiatric conditions, including Alzheimer's disease, bipolar disorder, and attention-deficit/hyperactivity disorder (ADHD). Targeting SORCS2 with a well-validated recombinant antibody allows researchers to study its expression levels, localization, and interaction partners in various experimental systems.

Product Specifications and Formulation Details

The Recombinant SORCS2 Antibody (Ab351395) from Aladdin Scientific is supplied in PBS (phosphate-buffered saline) only, without additional carrier proteins or preservatives unless otherwise stated on the datasheet. This PBS-only format offers flexibility for downstream conjugation, custom reformulation, or direct use in sensitive assays where extraneous proteins such as BSA could interfere. The recombinant format (absea#RN-085 series) ensures a defined, sequence-confirmed binding domain, delivering superior lot-to-lot reproducibility compared to conventional hybridoma-derived antibodies. Researchers should consult the product datasheet for confirmed host species, isotype, molecular weight of the target, and validated application-specific dilution ranges.

Recommended Storage and Handling

Because the Recombinant SORCS2 Antibody is formulated in PBS only (no stabilising carrier protein), proper storage is critical to preserve activity:

• Short-term (up to 1 month): Store at 4 °C. Avoid repeated freeze–thaw cycles.
• Long-term: Aliquot into single-use volumes and store at −20 °C or −80 °C. Adding a carrier protein such as 0.1–1% BSA prior to freezing is recommended if conjugation is not intended, to prevent non-specific adsorption to tube walls.
• Handling: Thaw on ice, mix gently, and centrifuge briefly before use. Do not vortex.

Always refer to the official product datasheet for the manufacturer's definitive storage recommendations, as conditions may be updated with lot-specific data.

Troubleshooting Common Issues

Researchers using antibodies in a PBS-only formulation may encounter the following issues and suggested remedies:

• High background signal: Ensure adequate blocking (e.g., 5% non-fat milk or BSA in TBST for Western blot). Optimise antibody dilution empirically.
• Weak or no signal: Verify that the target species is compatible with this antibody. Check that antigen retrieval conditions (for IHC/ICC) are appropriate for the epitope. Confirm that the secondary antibody host matches the primary antibody host species.
• Non-specific bands (Western blot): Reduce primary antibody concentration; increase wash stringency. Include a negative control (non-expressing cell line or knockdown/knockout sample).
• Precipitation or aggregation: Centrifuge the antibody at 10,000 × g for 5 minutes before use. Avoid freeze–thaw in the absence of a carrier protein.

If issues persist, contact Aladdin Scientific technical support with lot number, protocol details, and images for expert assistance.

Ordering, Compliance, and Support

The Recombinant SORCS2 Antibody is available exclusively for research use only (RUO) and is not intended for diagnostic or therapeutic applications in humans. To order:

1. Search SKU Ab351395 on the Aladdin Scientific website or contact your regional distributor.
2. Request a Safety Data Sheet (SDS) and Certificate of Analysis (CoA) for your lot before use.
3. For bulk quantities, OEM supply, or custom conjugation inquiries, contact Aladdin Scientific sales directly.

Returns and refunds are subject to Aladdin Scientific's standard return policy. For cold-chain reagents, please inspect shipments upon arrival and report any temperature excursion or damage within the specified claim window.

Frequently asked questions

What applications is the Recombinant SORCS2 Antibody (Ab351395) validated for?

The validated applications for Ab351395 should be confirmed on the official product datasheet, as they depend on the specific clone and lot. Typical applications for recombinant antibodies of this class include Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA; however, researchers should verify which applications are specifically stated for this product before use.

Why is the SORCS2 Antibody supplied in PBS only, and does this affect how I store or use it?

The PBS-only formulation provides maximum flexibility for researchers who wish to conjugate the antibody to a label, incorporate it into a custom buffer system, or avoid potential interference from carrier proteins like BSA in sensitive assays. Because there is no stabilising carrier protein, it is especially important to aliquot the antibody into single-use volumes before freezing at −20 °C or −80 °C to prevent activity loss from repeated freeze–thaw cycles and non-specific adsorption.

Is the Recombinant SORCS2 Antibody suitable for use with human, mouse, and rat samples?

Reactivity across species (human, mouse, rat, or others) depends on the specific epitope targeted by this recombinant antibody clone. Please consult the Ab351395 product datasheet for the confirmed species reactivity. If cross-reactivity with additional species is required, Aladdin Scientific technical support can advise on sequence homology and likelihood of cross-reactivity.

How does the recombinant format of this antibody compare to conventional hybridoma-derived antibodies?

Recombinant antibodies are produced from a defined, sequence-confirmed expression construct, which eliminates the genetic drift and batch variability inherent in hybridoma cell lines. This means researchers can expect consistent performance from lot to lot, facilitating reproducibility in long-term studies. The recombinant format also enables precise engineering of the binding domain and Fc region for custom downstream applications.

What should I do if I receive the SORCS2 Antibody and suspect a temperature excursion during shipping?

Inspect the shipment immediately upon receipt, checking for any temperature indicator included in the packaging. Do not use the product if you have strong reason to believe cold-chain integrity was compromised. Document the condition of the packaging and contact Aladdin Scientific customer service with your order number, lot number, and photographic evidence within the claim window specified in our shipping policy so we can arrange a replacement or credit.

References

1. Glerup S, et al. SorCS2 regulates dopaminergic wiring and is processed into an apoptotic two-chain receptor in peripheral glia. Neuron. 2014.
2. Deinhardt K, et al. Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein. Proceedings of the National Academy of Sciences. 2006.
3. Aladdin Scientific. Product Datasheet: Recombinant SORCS2 Antibody, Ab351395. Aladdin Scientific Internal Documentation.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.