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Red Blood Cell (RBC) Lysis Buffer

Protocols

Red Blood Cell (RBC) Lysis Buffer

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve the following in 800ml distilled H2O

8.3g NH4Cl
1.0g KHCO3
1.8ml of 5% EDTA

2.Filter sterilize through 0.2um filter

3.qs to 1000ml with distilled H2O

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Red Blood Cell (RBC) Lysis Buffer" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/red-blood-cell-rbc-lysis-buffer-en.html

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