SNX11 Mouse mAb: Applications, Specificity & Protocol Guide

SNX11 Mouse mAb is a monoclonal antibody raised in mouse against human SNX11 (Sorting Nexin 11), designed to support researchers investigating endosomal sorting, protein trafficking, and related cellular processes.

Product SKU

Ab351082

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

SNX11 (Sorting Nexin 11)

Formulation

PBS only (no carrier protein or preservative indicated)

Host Species

Mouse

Antibody Class

Monoclonal

SNX11 Biology and Research Significance

Sorting Nexin 11 (SNX11) is a member of the sorting nexin (SNX) family, a diverse group of proteins unified by the presence of a phox homology (PX) domain that mediates binding to phosphoinositide-enriched endosomal membranes. SNX family members play critical roles in intracellular protein trafficking, endosomal sorting, and the regulation of receptor recycling or degradation pathways.

SNX11 has been implicated in the modulation of endosomal membrane dynamics and may interact with components of the retromer complex or other trafficking machinery. Research into SNX11 function contributes to our understanding of lysosomal biogenesis, signal transduction regulation through receptor downregulation, and potentially disease mechanisms where vesicular trafficking is disrupted.

Reliable detection tools such as SNX11 Mouse mAb (Ab351082) are therefore essential for elucidating SNX11 localization, expression levels across tissue types, and its interactions with endosomal compartments.

Antibody Specifications and Format

SNX11 Mouse mAb (Ab351082) is supplied as a monoclonal antibody raised in mouse against the SNX11 target. The product is formulated in PBS only, without added carrier proteins such as BSA or preservatives such as sodium azide, which makes it directly compatible with conjugation workflows and minimises interference in sensitive downstream assays.

Because the formulation contains no preservative, it is important to handle the antibody under aseptic conditions and to follow the manufacturer's recommended storage and aliquoting guidelines to maintain activity over time.

Key specifications at a glance:

• Catalog number: Ab351082
• Host: Mouse
• Antibody format: Monoclonal
• Formulation: PBS only
• Target: SNX11

Recommended Applications and Usage Protocol

Monoclonal antibodies with PBS-only formulation such as SNX11 Mouse mAb (Ab351082) are broadly suited to a range of standard immunological techniques. Researchers should validate the antibody for each specific application and sample type in their laboratory. Common applications for anti-SNX11 monoclonal antibodies include:

• Western Blot (WB): Detect endogenous SNX11 protein in cell or tissue lysates. Recommended starting dilution should be optimised empirically; typical starting ranges for monoclonal antibodies in WB are 1:500–1:2000.
• Immunofluorescence (IF): Visualise SNX11 subcellular localisation in fixed cells, particularly to examine endosomal compartments.
• Immunoprecipitation (IP): Enrich SNX11 and associated protein complexes from cell lysates for co-immunoprecipitation studies of trafficking machinery.
• Immunohistochemistry (IHC): Assess SNX11 expression in formalin-fixed, paraffin-embedded (FFPE) tissue sections, subject to optimisation.

General protocol notes: For WB, run reduced SDS-PAGE, transfer to PVDF or nitrocellulose membrane, block with 5% non-fat milk or BSA in TBST, incubate with primary antibody overnight at 4 °C, wash, and detect with appropriate HRP-conjugated anti-mouse secondary antibody. Optimise blocking buffer and antibody dilution empirically.

Storage, Handling, and Stability

Proper storage is critical to maintaining the binding activity of SNX11 Mouse mAb (Ab351082). As the product is formulated in PBS without stabilisers or preservatives, the following best practices are recommended:

• Short-term storage (up to 1 month): Store at 4 °C.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
• Avoid: Repeated freeze–thaw cycles; exposure to temperatures above 25 °C for prolonged periods; contamination with proteases.
• Carrier protein addition: If long-term stability is a concern and downstream applications are compatible, addition of BSA to a final concentration of 0.1–0.5% may be considered, although this may interfere with conjugation or certain assay formats.

Always centrifuge briefly before use if precipitation is suspected. Record lot number and date of receipt for traceability.

Frequently Asked Questions

See the FAQ section below for answers to the most common questions about SNX11 Mouse mAb (Ab351082), including cross-reactivity, dilution optimisation, and troubleshooting high background or weak signal in western blot experiments.

Frequently asked questions

What species reactivity is confirmed for SNX11 Mouse mAb (Ab351082)?

The antibody is raised in mouse against SNX11; confirmed species reactivity should be verified in the official datasheet for Ab351082. Human reactivity is typical for antibodies generated against human SNX11 sequences, but cross-reactivity with mouse, rat, or other species should be validated experimentally.

Why is SNX11 Mouse mAb formulated in PBS only, and does this affect storage?

PBS-only formulation means the antibody contains no carrier protein (e.g., BSA) or preservative (e.g., sodium azide), making it ideal for direct conjugation to labels or surfaces. However, it is less stable than formulations containing stabilisers, so aliquoting and storage at −20 °C or −80 °C is strongly recommended to prevent activity loss from repeated freeze–thaw cycles.

How should I optimise the working dilution of SNX11 Mouse mAb for western blot?

Start with a dilution range of 1:500 to 1:2000 in blocking buffer and run a dilution series on a positive-control lysate known to express SNX11. Adjust dilution, blocking conditions (milk vs. BSA), and incubation time based on signal-to-noise ratio. Always include a negative control to monitor non-specific binding.

Can SNX11 Mouse mAb be used for immunofluorescence to study endosomal localisation?

Monoclonal antibodies of this type are commonly employed in immunofluorescence applications to study subcellular localisation. For endosomal trafficking studies, co-staining with established endosomal markers (e.g., EEA1 for early endosomes, Rab7 for late endosomes) is recommended to confirm SNX11 compartmental localisation. Specific validation for IF should be confirmed using the Ab351082 datasheet.

References

1. Worby CA, Dixon JE. Sorting out the cellular functions of sorting nexins. Nat Rev Mol Cell Biol. 2002;3(12):919-931. doi:10.1038/nrm974
2. Cullen PJ, Steinberg F. To degrade or not to degrade: mechanisms and significance of endocytic recycling. Nat Rev Mol Cell Biol. 2018;19(11):679-696. doi:10.1038/s41580-018-0053-7
3. Aladdin Scientific. SNX11 Mouse mAb (Ab351082) product datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.