SOC Broth (Powder)

Directions per Liter:

Dissolve 34.46g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.

Note: This solution can be autoclaved; extended temperatures could cause the solution to darken, which will not affect product usefulness.

Component	g/liter
Dextrose•H2O [20mM]	3.96
MgSO4•7H2O	5
Sodium Chloride	0.5
Tryptone	20
Yeast Extract 5	5
	Total: 34.46g/Liter
	500g makes 14.7 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.

Homogeneous: Proprietary blending technology ensures complete mixing of components.